Evidence map›Paper›PMID 41701563›Full record

ArticleJournal of leukocyte biology2026

RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization.

Daniel H Kim, Ramizah Syahirah, Conwy Zheng, Chang Ding, Alan Y Hsu, Tyler Pikes, Zhaolun Liang, Sheng Liu, Linlin Li, Xiaoping Bao and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of leukocyte biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Daniel H KimDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Ramizah SyahirahDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Conwy ZhengDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Chang DingDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Alan Y HsuDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Tyler PikesDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Zhaolun LiangDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.
Sheng LiuDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, 410 W 10th Street, Indianapolis, IN 46202, USA.
Linlin LiWeldon School of Biomedical Engineering, Purdue University, 206 S Martin Jischke Dr, West Lafayette, IN 47907, USA.
Xiaoping BaoDavidson School of Chemical Engineering, Purdue University, 480 Stadium Mall Drive, West Lafayette, IN 47907, USA.
David UmulisWeldon School of Biomedical Engineering, Purdue University, 206 S Martin Jischke Dr, West Lafayette, IN 47907, USA.
Jun WanDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, 410 W 10th Street, Indianapolis, IN 46202, USA.
Qing DengDepartment of Biological Sciences, Purdue University, 915 Mitch Daniels Blvd., West Lafayette, IN 47907, USA.ORCID 0000-0002-9254-9951

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI Andrea L Kasinski · 1985 to 2026
$43.4M
Role of microRNAs in neutrophil migrationR35GM119787 · NIGMS · PURDUE UNIVERSITY · PI Qing Deng · 2016 to 2026
$4.9M
Engineer Biomimetic Microfluidic Models to Investigate and Reprogram Tumor Associated Neutrophils for Cancer TherapyR37CA265926 · NCI · PURDUE UNIVERSITY · PI Xiaoping Bao · 2022 to 2026
$1.7M
CAR neutrophils produced in vivo to remodel tumor microenvironment and treat glioblastomaR01CA293514 · NCI · PURDUE UNIVERSITY · PI Xiaoping Bao, Qing Deng · 2025 to 2026
$1.3M
Institute for Cancer ResearchNCI NIH HHS R01 CA293514NCI NIH HHS R37 CA265926NIGMS NIH HHS R35 GM119787NIH HHS P30 CA023168NIH HHS R01CA293514NIH HHS R35GM119787NIH HHS R37CA265926
6 · The paper itself

Abstract

Neutrophils are the first responders of our innate immune system, crucial for defense against various infections. The intricate regulation of neutrophil migration is essential for neutrophil function. However, a complete mechanistic understanding is missing. We previously performed a miRNA overexpression screen and identified miR-190 as a potent suppressor of neutrophil migration in zebrafish. Through a second round of small-scale screening using neutrophil-specific knockouts of putative miR-190 targets, we identified that rabep1 (encoding Rabaptin, RAB GTPase binding effector protein 1) is essential for neutrophil motility and chemotaxis in zebrafish. Re-expressing full-length Rabaptin, but not its truncation lacking the Rab4/Rab5 binding domain, rescued cell motility in the knockout. Knocking down RABEP1 in differentiated human leukemia (dHL-60) cells consistently reduced cell motility. RABAPTIN-deficient dHL-60 cells are defective with fast recycling, yet maintain a normal Rab5 GTP level. The RABAPTIN-deficient cells displayed reduced PAK phosphorylation and decreased F-actin levels, yet still appropriately polarized upon chemokine stimulation. Overexpression of dominant-negative Rab4 or Rab5 has a similar inhibitory effect on neutrophil migration. Our data suggest that RABAPTIN drives endosomal recycling, Rac activation, and leading-edge actin polymerization, providing significant insights into the role of the endocytic pathway in neutrophil motility.

Indexed as

ActinsEndosomesNeutrophilsVesicular Transport ProteinsZebrafish ProteinsAnimalsCell MovementHL-60 CellsHumansMicroRNAsPolymerizationrac GTP-Binding ProteinsZebrafishActinsMicroRNAsrac GTP-Binding ProteinsVesicular Transport ProteinsZebrafish Proteinsendocytic pathwayHL-60 cellsmiR-190Rabep1zebrafish

Identifiers

PMID41701563
PMCPMC12997562

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.