Evidence map›Paper›PMID 41699346›Full record

ArticleFunctional & integrative genomics2026

CircDCAF6 promotes myoblast differentiation by inhibiting the ubiquitination of ACTC1.

Liang Chengcheng, Yu Shengchen, Hina Sultana, Zhao Chunping, Cheng Gong, Sameerh Alsahafi, Safa H Qahl, Fatimah A Alqahtani, Dalal Alenizi, Dalal Alardan and 1 more

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Article in Functional & integrative genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Liang ChengchengCollege of Animal Science and Technology, Xinyang Agriculture and Forestry University, Xinyang, Henan, 464000, China.
Yu ShengchenCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Hina SultanaIntegrative Program in Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Zhao ChunpingCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Cheng GongCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Sameerh AlsahafiDepartment of Biology, Faculty of Science, Al-Baha University, P.O. Box 1988, Al-Baha, 65527, Kingdom of Saudi Arabia.
Safa H QahlDepartment of Biological Sciences, College of Science, University of Jeddah, Jeddah, 21589, Saudi Arabia.
Fatimah A AlqahtaniDepartment of Biology, College of Science, King Khalid University, Abha, 7044, Saudi Arabia.
Dalal AleniziDepartment of Biology, College of Science, University of Hail, Hail, 2440, Saudi Arabia.
Dalal AlardanDepartment of Biology, College of Science, University of Hail, Hail, 2440, Saudi Arabia.
Linsen ZanCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China. zanlinsen@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to investigate the role and molecular mechanism of the circular RNA circDCAF6, which is specifically highly expressed in the muscle tissue of Qinchuan cattle, during myoblast differentiation. Through cell experiments, it was found that the expression level of circDCAF6 first increased and then decreased during myoblast differentiation. Overexpression of circDCAF6 significantly promoted myotube formation and the expression of myogenic differentiation marker genes, while knockdown of circDCAF6 inhibited differentiation. RNA pull-down and mass spectrometry analysis identified ACTC1 as an interacting protein of circDCAF6. circDCAF6 promotes myogenic differentiation by inhibiting the ubiquitination modification of ACTC1, thereby maintaining its stability. Further research revealed that the ubiquitin ligase TRIM28 is a target protein of ACTC1 and participates in the regulation of ACTC1 ubiquitination by circDCAF6. This study elucidates the regulatory mechanism of the “circDCAF6-ACTC1-TRIM28” signaling axis in myogenic differentiation, provides new insights into the role of circRNA in muscle development regulation, Provide new insights into the regulation of muscle development and offer potential clues for future breeding research.

Indexed as

Cell DifferentiationMuscle DevelopmentMyoblastsRNA, CircularAnimalsCattleCell LineUbiquitinationRNA, CircularACTC1Cell differentiationCircDCAF6TRIM28Ubiquitination

Identifiers

PMID41699346

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.