Development and Application of SRCA for Rapid Detection of Pseudomonas aeruginosa in Bottled Drinking Water.

Lianbao Zheng, Jiahong Zhu, Xiaohong Zhang, Yunbin Yang, Hongxia Yang, Tong Hu, Weihong Zhong

Abstract read

In one paragraph

Article in Molecular biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Lianbao ZhengCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.

Jiahong ZhuCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.

Xiaohong ZhangLishui Institute for Quality Inspection and Testing, Lishui, China.

Yunbin YangChangxing County Food and Drug Inspection and Testing Centre, Huzhou, China.

Hongxia YangHuzhou Institute for Food and Drug Control, Huzhou, China.

Tong HuCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China. hutong1992@zjut.edu.cn.

Weihong ZhongCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.ORCID http://orcid.org/0000-0002-5673-2222

Funding

National Natural Science Foundation of China Grant No. 32402036Natural Science Foundation of Zhejiang Province LQ23C010004

6 · The paper itself

Abstract

Pseudomonas aeruginosa is a significant waterborne pathogen that poses substantial public health risks, particularly in bottled drinking water. Traditional methods for detecting P. aeruginosa are often time-consuming, expensive, and require complex equipment. This study developed a novel Saltatory Rolling Circle Amplification (SRCA) method for the rapid, sensitive, and specific detection of P. aeruginosa in bottled drinking water. The technique utilizes a simple water bath for isothermal DNA amplification and incorporates SYBR Green I for visual colorimetric detection, eliminating the need for complex post-amplification processes such as gel electrophoresis. The SRCA assay demonstrated high sensitivity with a detection limit of 9.1 × 10

Indexed as

Drinking WaterNucleic Acid Amplification TechniquesPseudomonas aeruginosaBenzothiazolesColorimetryDiaminesDNA, BacterialLimit of DetectionQuinolinesSensitivity and SpecificityWater MicrobiologyBenzothiazolesDiaminesDNA, BacterialDrinking WaterQuinolinesSYBR Green IColorimetric assayDrinking waterP. aeruginosa detectionRapid detectionSaltatory rolling circle amplification

Identifiers

PMID41699172

DOI10.1016/j.talanta.2022.123821

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.