Evidence map›Paper›PMID 41699034›Full record

ArticleScientific reports2026

Ciprofol attenuates cerebral Ischemia‒reperfusion injury in rats by inhibiting ferroptosis through upregulating AMPK.

Hanyu Zeng, Xinran Yu, Zhiwen Zheng, Zhuo Wang, Wei Dong, Jianhui Gan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hanyu ZengDepartment of Anesthesiology, Tangshan People's Hospital, North China University of Science and Technology, 65 Shengli Road, Lunan, Tangshan, 063000, Hebei, People's Republic of China.
Xinran YuDepartment of Anesthesiology, Tangshan People's Hospital, North China University of Science and Technology, 65 Shengli Road, Lunan, Tangshan, 063000, Hebei, People's Republic of China.
Zhiwen ZhengDepartment of Anesthesiology, Tangshan People's Hospital, North China University of Science and Technology, 65 Shengli Road, Lunan, Tangshan, 063000, Hebei, People's Republic of China.
Zhuo WangDepartment of Experimental Research Center, Tangshan People's Hospital, North China University of Science and Technology, Tangshan, 063000, China.
Wei DongDepartment of Neurosurgery, Tangshan People's Hospital, North China University of Science and Technology, Tangshan, 063000, China.
Jianhui GanDepartment of Anesthesiology, Tangshan People's Hospital, North China University of Science and Technology, 65 Shengli Road, Lunan, Tangshan, 063000, Hebei, People's Republic of China. ganjianhui666@163.com.ORCID http://orcid.org/0000-0002-4946-3554

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study assesses the neuroprotective effects and molecular mechanisms of ciprofol against cerebral ischemia-reperfusion injury (CIRI) in rats. From July 2023 and July 2024, fifty male SD rats were randomly divided into five groups: control, model, ciprofol (Ci), erastin (Era), and compound C (CC). The model was induced by MCAO/R. Control group received identical surgical interventions without filament insertion. Rats neurological deficits were quantified using modified Garcia JH scores. Histopathological changes were evaluated through Nissl staining and TTC‒determined infarct volume. Mitochondrial ultrastructure was observed by transmission electron microscopy. Biochemical analyses quantified malondialdehyde (MDA), iron content and inflammatory cytokines (IL-1β, IL-6, TNF-α). Western blotting measured AMPK phosphorylation and ferroptosis proteins (GPX4, ACSL4). Relative to the model group rats, the Ci rats exhibited elevated modified Garcia JH scores (P < 0.05) accompanied by attenuated neuronal/mitochondrial damage and diminished infarct areas. Biochemical analyses revealed significant reductions in MDA, inflammatory cytokines, and ACSL4 protein expression in Ci rats, concurrent with enhanced GPX4 levels and elevated p-AMPK/T-AMPK ratios (P < 0.05). Notably, Era specimens demonstrated GPX4 downregulation with corresponding ACSL4 and iron accumulation compared to the Ci rats. Furthermore, the CC rats displayed decreased p-AMPK/T-AMPK activation relative to Ci rats (P < 0.05). Ciprofol ameliorates CIRI in rats by inhibiting ferroptosis and inflammatory factor through upregulating AMPK.

Indexed as

AMP-Activated Protein KinasesBrain IschemiaFerroptosisNeuroprotective AgentsReperfusion InjuryAnimalsCoenzyme A LigasesCyclopropanesDisease Models, AnimalMaleMitochondriaPhospholipid Hydroperoxide Glutathione PeroxidaseRatsRats, Sprague-DawleyUp-Regulation(2-(1R)-1-cyclopropyl)ethyl-6-isopropyl-phenolAcsl4 protein, ratAMP-Activated Protein KinasesCoenzyme A LigasesCyclopropanesglutathione peroxidase 4, ratNeuroprotective AgentsPhospholipid Hydroperoxide Glutathione PeroxidaseAMPKCerebral Ischemia-Reperfusion injuryCiprofolFerroptosis

Identifiers

PMID41699034
PMCPMC13000322

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.