Bioorthogonal Click Chemistry for Antibody-Free Profiling of Acetylation, Propionylation, and Butyrylation in

Haley N Monacchio, Ritika S Shah, Christian F Montes, Grace Z Wang, Justin W Walley, Chelsey M VanDrisse

Abstract read

In one paragraph

Article in ACS infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Haley N MonacchioUniversity of Georgia, Department of Microbiology, 140 Cedar Street, Athens, Georgia 30602, United States.

Ritika S ShahUniversity of Georgia, Department of Genetics, 120 E Green Street, Athens, Georgia 30602, United States.

Christian F MontesIowa State University, Plant Pathology, Entomology and Microbiology, 2213 Pammel Drive, Ames, Iowa 50011, United States.ORCID 0000-0003-1249-2308

Grace Z WangCalifornia Institute of Technology, Division of Chemistry and Chemical Engineering, 221 Spalding Laboratory, Pasadena, California 91125, United States.

Justin W WalleyIowa State University, Plant Pathology, Entomology and Microbiology, 2213 Pammel Drive, Ames, Iowa 50011, United States.ORCID 0000-0001-7553-2237

Chelsey M VanDrisseUniversity of Georgia, Department of Genetics, 120 E Green Street, Athens, Georgia 30602, United States.ORCID 0000-0001-9139-0443

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lysine acylation is a posttranslational modification (PTM) conserved in all domains of life and is essential for regulating diverse biological processes. Traditional methods for investigating acylation rely on anti-acyl-lysine antibodies, which are costly and time-consuming and often exhibit variable affinity. To remedy these pitfalls, we developed an antibody-free method for bacterial acylome enrichment using bioorthogonal click chemistry coupled with tandem mass spectrometry. We applied this approach to the pathogens

Indexed as

Click ChemistryMethicillin-Resistant Staphylococcus aureusPseudomonas aeruginosaAcetylationAcylationBacterial ProteinsLysineProtein Processing, Post-TranslationalTandem Mass SpectrometryBacterial ProteinsLysineacetylationbacterial acylationbutyrylationposttranslational modificationspropionylation

Identifiers

PMID41697727

DOI10.1021/acsinfecdis.5c00985

PMCPMC12993845

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.