Evidence map›Paper›PMID 41697548›Full record

ArticleInflammopharmacology2026

Synergistic wound healing mechanisms of Heliotropium curassavicum extracts via redox modulation, inflammation suppression, and tissue remodeling: linking phytochemical diversity to antioxidant and anti-inflammatory effects.

Rania F Ahmed, Dalia M Rasheed, Noha A Mowaad, Rania Elgohary, Eman H Eltantawy, Eman A Negm, Mohamed A Farag, Abdelsamed I Elshamy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammopharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rania F AhmedDepartment of Natural Compounds Chemistry, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.
Dalia M RasheedPharmacognosy Department, Faculty of Pharmacy, October 6 University, Central Axis, Part 1/1, Sixth of October, Giza, Egypt.
Noha A MowaadNarcotics, Ergogenics and Poisons Department, Medical Research and Clinical Studies Institute, National Research Centre, P.O.12622, Dokki, Giza, Egypt.
Rania ElgoharyNarcotics, Ergogenics and Poisons Department, Medical Research and Clinical Studies Institute, National Research Centre, P.O.12622, Dokki, Giza, Egypt.
Eman H EltantawyDepartment of Medical Histology, Faculty of Medicine, Ain Shams University, Cairo, 1181, Egypt.
Eman A NegmDepartment of Medical Histology, Faculty of Medicine, Ain Shams University, Cairo, 1181, Egypt.
Mohamed A FaragPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr el Aini St., Cairo, 11562, Egypt.
Abdelsamed I ElshamyDepartment of Natural Compounds Chemistry, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt. elshamynrc@yahoo.com.ORCID http://orcid.org/0000-0003-3302-3623

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study assessed the wound-healing potential of Heliotropium curassavicum via a comparative metabolite profiling of its ethanol (HC-EtOH), ethyl acetate (HC-EtOAc), and n-butanol (HC-BuOH) extracts, alongside in vivo assessment. UHPLC-ESI-MS/MS analysis annotated 86 metabolites, including 13 pyrrolizidine alkaloids (4 of which, viz. uplandicine, uluganine, curassavine, and curassavine N-oxide are newly reported in the genus), 12 phenylpropanoids, and 8 fatty acid amides. Topical application of the extracts accelerated wound closure in a dose-dependent manner, with HC-BuOH exhibiting the strongest activity. HC-BuOH (200 mg/kg) achieved near-complete closure by day 14 and improved healing by 39% compared with Intrasite Gel. This was accompanied by significant modulation of oxidative and inflammatory markers, including a 66% reduction in MDA, a 220% increase in GSH, a 94% reduction in TNF-α, and restoration of PGE-2 toward normal levels (p < 0.05). Histopathology confirmed complete re-epithelialization, organized collagen deposition, and localized Ki-67 expression in HC-BuOH–treated wounds. Multivariate data and interaction analyses, supported by PLSR model demonstrated that the superior wound-healing activity of HC-BuOH is attributed for synergistic interactions among pyrrolizidine alkaloids, phenylpropanoids, and fatty acyl amides. These findings highlight H. curassavicum, particularly the HC-BuOH fraction, as a promising natural wound-healing agent. These effects are likely attributed to plant richness, in pyrrolizidine alkaloids, phenylpropanoids, and fatty acyl amides, highlighting its potential as a natural wound healing agent.

Indexed as

Anti-Inflammatory AgentsAntioxidantsHeliotropiumInflammationPhytochemicalsPlant ExtractsWound HealingAnimalsDose-Response Relationship, DrugMaleOxidation-ReductionRatsAnti-Inflammatory AgentsAntioxidantsPhytochemicalsPlant ExtractsAnti-inflammatoryAntioxidant activityHeliotropium curassavicumMetabolite profilingPyrrolizidine alkaloidsWound healing

Identifiers

PMID41697548
PMCPMC12996408

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.