Evidence map›Paper›PMID 41697360›Full record

ArticleEuropean journal of trauma and emergency surgery : official publication of the European Trauma Society2026

In-vitro evaluation of the proliferative and osteogenic activity of atrophic non-union derived mesenchymal stem cells compared to autologous bone graft derived mesenchymal stem cells.

Tim Niklas Bewersdorf, Jakob Hofmann, Laura Boehm, Sebastian Findeisen, Christian Schamberger, Thomas Lingner, Ulrike Sommer, Gerhard Schmidmaier, Tobias Grossner

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of trauma and emergency surgery : official publication of the European Trauma Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tim Niklas BewersdorfFaculty of Medicine, Heidelberg University, Heidelberg, 69120, Germany.ORCID http://orcid.org/0000-0002-1188-4791
Jakob HofmannHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany.ORCID http://orcid.org/0009-0005-9018-9971
Laura BoehmHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany.
Sebastian FindeisenHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany.ORCID http://orcid.org/0000-0003-4083-1311
Christian SchambergerHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany.ORCID http://orcid.org/0000-0002-9121-472X
Thomas LingnerGenevention GmbH, Rudolf-Wissell-Str. 28A, Göttingen, 37079, Germany.
Ulrike SommerHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany.ORCID http://orcid.org/0009-0002-6698-0884
Gerhard SchmidmaierHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany.ORCID http://orcid.org/0000-0002-6711-7380
Tobias GrossnerHeidelberg Trauma Research Group (HTRG), Clinic for Trauma and Reconstructive Surgery, Centre for Orthopaedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Heidelberg, 69120, Germany. tobias.grossner@med.uni-heidelberg.de.ORCID http://orcid.org/0000-0001-8953-9384

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study compares the proliferative and osteogenic activity of human bone marrow derived mesenchymal stem cells (MSCs) obtained from atrophic non-unions and from healthy autologous bone graft tissue (harvested from iliac crest or femoral canal) of the same patient in an in-vitro setting utilizing a matched control study design.

methodsMSCs underwent osteogenic differentiation over 3 weeks in-vitro (n = 6 donors; n = 36 samples/group) and the proliferative activity was accessed using DAPI-based immunofluorescence microscopy and WST-1 assay. All results regarding the osteogenic activity were normalized to 10

resultsThe early and late-stage osteogenic activity of atrophic non-union MSCs was significantly higher compared to healthy control graft MSCs on day 21 of osteogenic differentiation in-vitro, both in absolute numbers (early/late: p < 0.001) and after normalization to 10

conclusionThe results emphasize that the in-vitro osteogenic and proliferative activity of atrophic non-union MSCs is not impaired as clinically assumed but the osteogenic potential of atrophic non-union MSCs is in fact significantly higher compared to graft derived MSCs. This might be an important basic-science insight for the optimization of clinical non-union therapies.

Indexed as

Bone TransplantationCell ProliferationFractures, UnunitedMesenchymal Stem CellsOsteogenesisAdultAlkaline PhosphataseCell DifferentiationCells, CulturedFemaleHumansIliumMaleMiddle AgedTransplantation, AutologousAlkaline PhosphataseAtrophic non-unionAutologous bone graftBone-marrow mesenchymal stem cellsIn-vitroOsteogenic activityProliferative activity

Identifiers

PMID41697360
PMCPMC12909382

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.