Evidence map›Paper›PMID 41696448›Full record

ArticleInternational journal of analytical chemistry2026

A High-Performance Liquid Chromatography Method for Simultaneous Analysis of Naringin, Ascorbic Acid, and Caffeine: Design, Development, and Validation.

Murad Abualhasan, Mohammad Mousa, Hitaf Izhiman, Malak Alkhatib, Ibaa Aghbar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Murad AbualhasanFaculty of Pharmacy, An-Najah National University, P.O. Box 7, Nablus, State of Palestine, najah.edu.ORCID https://orcid.org/0000-0002-2083-856X
Mohammad MousaFaculty of Pharmacy, An-Najah National University, P.O. Box 7, Nablus, State of Palestine, najah.edu.
Hitaf IzhimanFaculty of Pharmacy, An-Najah National University, P.O. Box 7, Nablus, State of Palestine, najah.edu.
Malak AlkhatibFaculty of Pharmacy, An-Najah National University, P.O. Box 7, Nablus, State of Palestine, najah.edu.
Ibaa AghbarFaculty of Pharmacy, An-Najah National University, P.O. Box 7, Nablus, State of Palestine, najah.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The simultaneous quantification of ascorbic acid, caffeine, and naringin is of significant interest due to their potential therapeutic applications, particularly in the prevention and management of colorectal cancer. While numerous HPLC methods exist for the individual determination of each compound, our method stands out for its reliability in addressing all three compounds in a single analytical run. This study aimed to develop and validate a robust reverse-phase HPLC method for the simultaneous analysis of these compounds in accordance with ICH Q2(R1) and USP guidelines. A robust reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous determination of ascorbic acid, caffeine, and naringin in the concentration range of 7.813-125 μg/mL. Chromatographic separation was achieved on a Symmetry Shield RP-18 column (250 × 4.6 mm, 5 μm) using an isocratic mobile phase of 75% phosphate buffer (pH 3.5) and 25% acetonitrile at a flow rate of 1 mL/min, with detection at 220 nm. Method validation confirmed specificity, selectivity, and excellent linearity (

Identifiers

PMID41696448
PMCPMC12903793

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.