Effects of guanidinoacetic acid on gene expression in adipose tissue of broiler chickens: a differential expression analysis based on small RNA sequencing.

Mengqian Liu, Jinrui Ruan, Yu Yang, Mengyuan Li, Zifu Gu, Changrong Ge, Weina Cao

Abstract read

In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Effects of rumen-protected guanidinoacetic acid on fat deposition, antioxidant capacity, fatty acid profiles in subcutaneous adipose of Tibetan sheep: integrated RNA-seq and LC-MS/MS analysis.Frontiers in veterinary science · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mengqian Liu *College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Jinrui Ruan *College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Yu Yang *College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Mengyuan LiCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Zifu GuCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Changrong GeCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Weina CaoCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to elucidate the mechanism by which guanidinoacetic acid (GAA) reduces abdominal fat deposition in broilers, focusing on the regulatory role of microRNAs (miRNAs). Excessive fat accumulation in the abdomen is a common issue in meat-type chickens as it reduces feed efficiency and compromises meat quality. By performing small RNA (sRNA) sequencing and analysis on broilers from GAA-treated and control groups, a total of 46 differentially expressed miRNAs were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that these miRNAs were significantly enriched in pathways related to lipid metabolism. Further investigation revealed that GAA likely reduces fat deposition and promotes lipid oxidation by up-regulating gga-miR-103-3p and gga-miR-107-3p, thereby inhibiting their target genes, stearoyl-CoA desaturase (SCD) and transmembrane protein 35B (TMEM35B). These findings provide a theoretical foundation for the application of GAA to improve growth performance and meat quality in broilers.

Indexed as

adiposebroilerfat depositionguanidinoacetic acidmicroRNAtranscriptome

Identifiers

PMID41695215

DOI10.3389/fvets.2025.1732976

PMCPMC12894009

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.