ArticleVeterinarni medicina2026
Dairy bull sperm subpopulation behaviour in frozen-thawed semen across breed, temperature, and thawing time.
Article in Veterinarni medicina, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Optimising dairy cattle reproduction with assisted reproductive technologies, such as artificial insemination, requires standardised semen handling and analysis. This study evaluated the kinematic structure of the spermatozoan subpopulation in frozen-thawed Holstein and Jersey bull semen doses under different thawing protocols. We used frozen semen doses taken from four bulls of each breed. Nine semen doses were collected from each animal, for a total of 72 cryopreserved doses. Straw thawing was performed at three temperatures (35 °C, 37 °C, and 40 °C) and three times (30 s, 40 s, and 45 s). Sperm kinematic patterns were evaluated using a CASA-mot system (ISAS
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