ArticleAnatomia, histologia, embryologia2026
Beyond IHC Stain: Mitochondria as a Histochemical Biomarker for Apoptosis Detection in Hepatic Tissue Sections.
Article in Anatomia, histologia, embryologia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Beyond IHC Stain: Mitochondria as a Histochemical Biomarker for Apoptosis Detection in Hepatic Tissue Sections.Anatomia, histologia, embryologia · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apoptosis plays a critical role in maintaining tissue homeostasis and preventing pathological conditions. The accurate detection of apoptosis is crucial for both research and diagnostic pathology, yet conventional histological staining methods often lack the sensitivity to identify early apoptotic changes. This study aimed to evaluate and compare the effectiveness of classical and emerging mitochondria-targeting histological techniques in detecting apoptotic hepatocytes. Paraffinated hepatic tissue from five rabbits was analysed using haematoxylin-eosin (H&E), Goldner's trichrome (GT), immunohistochemistry for caspase-3 (IHC-Casp3) and Heidenhain's iron haematoxylin (HIH) staining. The percentage of apoptotic hepatocytes was quantified using two assessment methods, and statistical analyses determined the sensitivity of staining protocols. Histologically, the main apoptotic features identified in HIH-stained hepatic specimens were fading 'ghost' mitochondria throughout the cytoplasm, chromatin condensation, fragmentation and dissolution and nucleolar margination. A similar proportion of apoptotic hepatocytes on the HIH staining compared to IHC-Casp3 (19.00% ± 0.7 for HIH vs. 18.50% ± 0.7 for IHC-Casp3) was observed. In comparison, significantly lower values were obtained in H&E (5.75%) and GT (7.5%) stains. The lack of statistical significance of HIH vs. IHC-Casp3 demonstrates similar sensitivities. The additional quantitative analysis methods confirmed IHC as the most sensitive method (3.36 ± 3.82 apoptotic cells/field), followed by HIH, H&E, and GT. In situ histological evaluation of apoptosis remains challenging in standard H&E and GT-stained sections. HIH stain, as a cost-effective alternative to IHC, highlights early stages of apoptotic cells, providing a significant advantage over classical staining methods, emphasising their importance in diagnostic histopathology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.