Evidence map›Paper›PMID 41692804›Full record

ArticleScientific reports2026

Novel marker genes for simultaneous detection of Salmonella, EHEC O157:H7, and Cronobacter.

Huina Zhang, Peng Xiong, Zhi Lu, Qingyong Zhang, Xinlu Chen, Ruhua Yan, Lei Wang, Xiaowei Tong, Tianyi Li, Lili Zou and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Huina Zhang *Hubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Peng Xiong *Hubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Zhi Lu *Hubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Qingyong ZhangThe First College of Clinical Medical Science, Department of Clinical Laboratory of Yichang Central People's Hospital, China Three Gorges University, Yichang, 443002, Hubei, China.
Xinlu ChenHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Ruhua YanHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Lei WangHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Xiaowei TongThe Third People's Hospital of Yichang, Yichang, 443002, Hubei, China.
Tianyi LiHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Lili ZouHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China.
Long YuHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China. yulong@ctgu.edu.cn.
Lu WangHubei Key Laboratory of Tumor Microenvironment and ImmunoTherapy, School of Basic Medicine, China Three Gorges University, Yichang, 443002, Hubei, China. wanglou@ctgu.edu.cn.

Funding

The National Natural Science Foundation of China ; The Natural Science Foundation of Hubei Province ; The Open Foundation of Hubei Province Key Laboratory of Tumor Microenvironment and Immunotherapy (China) (No. 32201986); (No. 2022CFB743, 2024AFD145); (No. 2024ZLKF2-27, 2024ZLKF2-51)
6 · The paper itself

Abstract

Food-borne pathogens represent a significant threat to global food safety and public health. Enterohemorrhagic Escherichia coli (EHEC) O157:H7, Salmonella, and Cronobacter are among the principal bacterial agents responsible for food-borne illnesses. Advances in sequencing technology have made it possible to identify particular genetic markers for these pathogens through large-scale genomic analysis. In this study, two conserved genes, z0340 and sbcC, were screened in silico from extensive bacterial genomes (including 1,460,756 genomes) for EHEC O157:H7 and Salmonella, respectively. Based on these two newly identified markers, combined with previously identified Cronobacter marker gene ygcB, multiplex PCR and multiplex TaqMan quantitative PCR methods were developed and established for the simultaneous detection of EHEC O157:H7, Salmonella, and Cronobacter. The specificity of the multiplex PCR and multiplex TaqMan qPCR was determined using 15 strains of Cronobacter, one strain of EHEC O157:H7, seven strains of Salmonella, and 100 strains of other common environmental bacteria and pathogens. The results showed that two methods demonstrated 100% specificity, both for multiple PCR and TaqMan qPCR. The detection limits of the multiplex PCR and TaqMan qPCR assays were 1 pg/μL and 0.5 pg/μL, respectively. Therefore, the newly developed methods are sensitive and reliable for the simultaneous identification of the three common food-borne pathogens.

Indexed as

CronobacterEscherichia coli O157Genes, BacterialSalmonellaFood MicrobiologyGenetic MarkersMultiplex Polymerase Chain ReactionSensitivity and SpecificityGenetic Markers

Identifiers

PMID41692804
PMCPMC13002893

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.