Evidence map›Paper›PMID 41692260›Full record

ArticleJournal of virological methods2026

Comparison of whole-viral genome sequencing and PCR-based assay for anal HPV detection to inform clinical implementation.

Omar Harfouch, Derek MacMath, Poorani Subramanian, Rahwa Eyasu, Habib O Ramadhani, Peiying Ye, Ashley Davis, Meredith Zoltick, Emade Ebah, Amay Shah and 13 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Omar HarfouchUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA. Electronic address: oharfouch@ihv.umaryland.edu.
Derek MacMathNational Institute of Allergy and Infectious Diseases (NIAID), Bethesda, MD, USA.
Poorani SubramanianBioinformatics and Computational Biosciences Branch, Office of Cyber Infrastructure and Computational Biology, NIAID, Bethesda, MD, USA.
Rahwa EyasuUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Habib O RamadhaniUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Peiying YeNational Institute of Allergy and Infectious Diseases (NIAID), Bethesda, MD, USA.
Ashley DavisUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Meredith ZoltickUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Emade EbahUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Amay ShahNational Institute of Allergy and Infectious Diseases (NIAID), Bethesda, MD, USA.
Amelia CoverUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Phyllis BijoleHIPS Clinic, Washington DC, USA.
Rachel SilkUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
David SternbergUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Tina LiuNational Institute of Health Clinical Center - Critical Care Medicine Department, Bethesda, MD, USA.
Grace GarrettNational Institute of Health Clinical Center - Critical Care Medicine Department, Bethesda, MD, USA.
Miriam JonesHIPS Clinic, Washington DC, USA.
Randy KierHIPS Clinic, Washington DC, USA.
Henry MasurNational Institute of Health Clinical Center - Critical Care Medicine Department, Bethesda, MD, USA.
Shyamasundaran KottililUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Sarah KattakuzhyUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Elana S RosenthalUniversity of Maryland Baltimore, School of Medicine, Institute of Human Virology, Baltimore, MD, USA.
Andrea LiscoNational Institute of Allergy and Infectious Diseases (NIAID), Bethesda, MD, USA.

Funding

The Role of Sex-Hormones in Anal Human Papilloma Virus Persistence (HPV) and Systemic and Mucosal Immunity in people with or at risk for HIVK23AI189218 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI Omar Harfouch · 2025 to 2026
$289k
NIAID NIH HHS K23 AI189218
6 · The paper itself

Abstract

To optimize anal cancer screening strategies, we compared two molecular platforms for anal high-risk Human Papillomavirus (HR-HPV) detection: whole-viral genome sequencing (WGS) and Cobas HPV-test-PCR (RC). Anal swabs from participants at high-risk for HPV acquisition were evaluated for cytological abnormalities and HR-HPV prevalence. Compared to WGS, RC had 51 % sensitivity and 98 % specificity for HPV16, 29 % and 92 % for HPV18, and 74 % and 93 % for non-16/18 HR-HPV. In a population with high prevalence of anal HR-HPV, RC had lower sensitivity in the detection of anal HR-HPV but was more robustly associated with abnormal anal cytology compared to WGS. In addition, WGS-based identification of HPV16 sub-lineages demonstrated that the composite A1, A2, A4, and C1 sub-lineages were associated with a higher risk of abnormal cytology compared to other HPV16 sub-lineages. WGS can further delineate the natural history of anal HR-HPV and their sub-lineages in populations at high-risk for HPV acquisition.

Indexed as

Anal CanalGenome, ViralHuman Papillomavirus VirusesPapillomaviridaePapillomavirus InfectionsPolymerase Chain ReactionWhole Genome SequencingAdultAgedAnus NeoplasmsDNA, ViralEarly Detection of CancerFemaleHumansMaleMiddle AgedDNA, ViralAnal CancerHPVNext-generation sequencing

Identifiers

PMID41692260
PMCPMC13218315

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.