Evidence map›Paper›PMID 41691811›Full record

ArticlePoultry science2026

ANKRD9 negatively regulates chicken myogenesis through ubiquitin-mediated regulation of IMPDH2.

Yuanfang Li, Pengtao Yuan, Jing Wang, Shuaihao Li, Bin Zhai, Hongtai Li, Zhilei Li, Lanya Wang, Yujie Guo, Wei Wang and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yuanfang LiSchool of Medicine and Health, Harbin Institute of Technology, Harbin, HeiLongJiang, 150001, China; Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou, Henan, 450000, China; The Shennong Laboratory, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Pengtao YuanHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China; Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China.
Jing WangSchool of Medicine and Health, Harbin Institute of Technology, Harbin, HeiLongJiang, 150001, China; Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou, Henan, 450000, China.
Shuaihao LiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Bin ZhaiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Hongtai LiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Zhilei LiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Lanya WangHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Yujie GuoHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Wei WangHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Yanhua ZhangHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Shuangli ZhuSchool of Medicine and Health, Harbin Institute of Technology, Harbin, HeiLongJiang, 150001, China; Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou, Henan, 450000, China.
Yingying LiuHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Zhuanjian LiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Yadong TianHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Xiangtao KangThe Shennong Laboratory, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China.
Yadong WangSchool of Medicine and Health, Harbin Institute of Technology, Harbin, HeiLongJiang, 150001, China; Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou, Henan, 450000, China.
Guoxi LiThe Shennong Laboratory, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Henan Agricultural University, Zhengzhou 450046, China. Electronic address: liguoxi0914@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Skeletal muscle development is regulated by transcriptional and post-translational mechanisms. While ankyrin repeat proteins participate in post-translational modifications, their role in myogenesis remains unclear. This study identifies ankyrin repeat domain-containing protein 9 (ANKRD9) as a novel negative regulator of chicken skeletal muscle development. ANKRD9 showed dynamic expression during postnatal muscle growth and downregulated cell cycle and DNA replication-related genes. Functionally, ANKRD9 overexpression inhibited myoblast proliferation and differentiation, while its knockdown enhanced these processes. In vivo, siRNA-mediated knockdown of ANKRD9 markedly increased muscle mass and myofiber diameter in chicks. Mechanistically, ANKRD9 bound directly to inosine monophosphate dehydrogenase 2 (IMPDH2), a rate-limiting enzyme in purine synthesis, and promoted its ubiquitin-mediated degradation without affecting mRNA levels. Crucially, rescue experiments confirmed that restoring IMPDH2 expression effectively reversed the inhibitory effects of ANKRD9 on myoblast proliferation and differentiation. Thus, this study unveils a novel regulatory axis in which ANKRD9 negatively regulates skeletal myogenesis by mediating the ubiquitination of IMPDH2. This discovery not only provides new insights into the post-translational regulatory network governing muscle development but also offers a potential target for genetic improvement of meat yield in poultry.

Indexed as

Avian ProteinsChickensIMP DehydrogenaseMuscle DevelopmentMuscle ProteinsAnimalsCell DifferentiationMuscle, SkeletalMyoblastsUbiquitinAvian ProteinsIMP DehydrogenaseMuscle ProteinsUbiquitinMeat yieldMyoblast differentiationMyoblast proliferationSkeletal muscle developmentUbiquitination

Identifiers

PMID41691811
PMCPMC12925506

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.