Evidence map›Paper›PMID 41691420›Full record

ArticleAdvanced healthcare materials2026

Purpose-Adaptable Reinforced 3D Hyaluronic-Acid Based Platform to Study Pathomechanisms of the Central Nervous System.

Nicoletta Murenu, Esra Tuerker, Anna-Lena Wiessler, Jessica Faber, Ievgenii Liashenko, Jeanette Weigelt, Jörg Tessmar, Paul D Dalton, Sibylle Jablonka, Mateo S Andrade Mier and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Nicoletta MurenuInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0009-0003-0905-2288
Esra TuerkerInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0000-0002-9328-5513
Anna-Lena WiesslerInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0000-0002-2039-3919
Jessica FaberDepartment of Mechanical Engineering, Institute of Continuum Mechanics and Biomechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Fürth, Germany.ORCID https://orcid.org/0000-0001-8372-495X
Ievgenii LiashenkoPhil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, Oregon, USA.ORCID https://orcid.org/0000-0002-1238-8327
Jeanette WeigeltDepartment of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Würzburg, Germany.
Jörg TessmarDepartment of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Würzburg, Germany.
Paul D DaltonPhil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, Oregon, USA.ORCID https://orcid.org/0000-0001-9602-4151
Sibylle JablonkaInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0000-0002-4517-3760
Mateo S Andrade MierInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0009-0003-5153-0693
Carmen VillmannInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0000-0003-1498-6950
Silvia BuddayDepartment of Mechanical Engineering, Institute of Continuum Mechanics and Biomechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Fürth, Germany.ORCID https://orcid.org/0000-0002-7072-8174
Natascha SchaeferInstitute for Clinical Neurobiology, University Hospital of Würzburg, Würzburg, Germany.ORCID https://orcid.org/0000-0001-9743-1963

Funding

"Biofabrication"Deutsche Forschungsgemeinschaft SFB/TRR225(subprojects A02, A07, B09, C05) 326 998 133-TRR 225
6 · The paper itself

Abstract

Three-dimensional (3D) models to study human disease mechanisms have demonstrated that the third dimension is an essential component for neuronal maturation and function. However, 3D neuronal cell culture is challenging due to their ultra-soft nature and specific extracellular matrix (ECM) organization. This study presents a microfiber reinforcement approach, combining primary mouse spinal cord neurons (SCNs) in a purpose-adaptable hyaluronic-acid-based matrix with melt electrowritten (MEW) frames to study disease mechanisms. The importance of laminins (LNs) is evaluated, which are vital for neuronal adhesion and maturation. Astrocytes (ACs) are mandatory in brain development and function by secretion of signal molecules, maintaining ion homeostasis, clearing of neurotransmitters, and by actively modulating neuronal activity. Three combinations are compared (i) isolated primary SCN, (ii) SCN with ACs (SCN-AC) and (iii) SCN-AC and LNs (SCN-AC-LN). Multimodal analysis by comparing protein expression, dendrite length, complex mechanical properties, and network functionality via Ca

Indexed as

Central Nervous SystemHyaluronic AcidAnimalsAstrocytesCell Culture Techniques, Three DimensionalCells, CulturedExtracellular MatrixHumansMiceNeuronsSpinal CordHyaluronic Acid3D disease model3D spinal cord modelautoimmune diseasehyaluronic acidinhibitory synapselaminin

Identifiers

PMID41691420
PMCPMC13175302

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.