Evidence map›Paper›PMID 41691100›Full record

ArticleCommunications biology2026

Exploring microbial diversity using cell-size fractionated enrichment incubations from subsurface aquifers at Äspö, Sweden.

George Westmeijer, Stephanie Turner, Patrik Hevele, Maliheh Mehrshad, Stefan Bertilsson, Mark Dopson

Abstract read
In one paragraph

Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

George WestmeijerCentre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4, Kalmar, Sweden.ORCID http://orcid.org/0000-0002-5529-2237
Stephanie TurnerCentre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4, Kalmar, Sweden.ORCID http://orcid.org/0009-0007-1915-0139
Patrik HeveleCentre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4, Kalmar, Sweden.
Maliheh MehrshadDepartment of Aquatic Sciences and Assessment, Science for Life Laboratory, Swedish University of Agricultural Sciences, Uppsala, Sweden.ORCID http://orcid.org/0000-0002-1108-6888
Stefan BertilssonDepartment of Aquatic Sciences and Assessment, Science for Life Laboratory, Swedish University of Agricultural Sciences, Uppsala, Sweden.ORCID http://orcid.org/0000-0002-4265-1835
Mark DopsonCentre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4, Kalmar, Sweden. mark.dopson@lnu.se.ORCID http://orcid.org/0000-0002-9622-3318

Funding

Vetenskapsrådet (Swedish Research Council) 2017-04422Vetenskapsrådet (Swedish Research Council) 2018-04311Vetenskapsrådet (Swedish Research Council) 2024-04607
6 · The paper itself

Abstract

The continental subsurface hosts energy-constrained groundwaters with a high diversity of ecologically elusive microorganisms adapted to the prevailing low-energy conditions. This study explored potential interactions among microbes using anaerobic enrichment incubations with three types of groundwater of contrasting hydrochemistry from the Äspö Hard Rock Laboratory, Sweden. Removing cells larger than 0.45 µm from the inoculum resulted in incubations enriched in populations characterized by very small genomes, including Patescibacteria, Nanobdellota, and Omnitrophota. These incubations had a higher diversity than non-fractionated incubations. However, cell numbers and community structure of the fractionated incubations did not change over an incubation period up to four months, despite high microbial diversity and experimental amendments with either simple (acetate) or more complex (cell lysate) carbon sources. In addition, network analysis on the groundwaters revealed multiple co-occurrences between populations affiliated with the Patescibacteria and the Desulfobacterota. Overall, these findings support that a considerable part of microbial diversity has a small cell size in these low energy groundwaters and strong co-occurrences among populations as an important survival strategy.

Indexed as

BacteriaBiodiversityGroundwaterWater MicrobiologyPhylogenySweden

Identifiers

PMID41691100
PMCPMC12992704

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.