Evidence map›Paper›PMID 41690962›Full record

ArticleScientific reports2026

The bacterial community composition of American Lobster (Homarus americanus) embryos and recently hatched larvae held under experimental laboratory conditions.

J Sarah Koshak, Bongkeun Song, Brittany Jellison, Abigail R Sisti, Emily B Rivest, Jeffrey D Shields

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

J Sarah KoshakVirginia Institute of Marine Science, William & Mary, Gloucester Point, VA, 23062, USA.
Bongkeun SongVirginia Institute of Marine Science, William & Mary, Gloucester Point, VA, 23062, USA.
Brittany JellisonVirginia Institute of Marine Science, William & Mary, Gloucester Point, VA, 23062, USA.
Abigail R SistiVirginia Institute of Marine Science, William & Mary, Gloucester Point, VA, 23062, USA.
Emily B RivestVirginia Institute of Marine Science, William & Mary, Gloucester Point, VA, 23062, USA.
Jeffrey D ShieldsVirginia Institute of Marine Science, William & Mary, Gloucester Point, VA, 23062, USA. jeff@vims.edu.

Funding

NOAA NA19OAR4170393NOAA NA24OARX417C0578
6 · The paper itself

Abstract

Previous research investigating the microbial community of American lobster embryos has long led researchers to believe this habitat comprised only a select few bacterial taxa. However, using 16 S rRNA gene sequencing, we show this community to be more diverse than previously thought. We have characterized the microbiome on lobster embryos held in a long-term laboratory experiment, investigated how the bacterial communities of American lobster embryos and larvae changed over embryogenesis and hatching in response to two environmental variables, and identified core members of the microbiome on embryos and larvae at two different developmental stages. Ovigerous female lobsters caught from Maine and Massachusetts were held under varying temperature and pH regimes that approximated observed and predicted warming and ocean acidification conditions in the Gulf of Maine (GoM) and Southern New England (SNE). The bacterial microbiome associated with the lobster embryos was quantified from two-time points during the 5-month experiment, as well as larvae collected within 12 h of hatching. Alpha diversity increased with each life history stage, and embryo and larvae microbiomes shared little community overlap with that in the surrounding tank water. Neither environmental conditions nor lobster origin significantly altered bacterial communities, with life history stage driving alpha and beta diversity. Embryos and larvae shared three core bacterial members identified as members of the genera Rubritalea, Delftia, and Stenotrophomonas. American lobster embryos and larvae appear to have a highly selective microhabitat for bacteria that showed little alteration by environmental conditions in laboratory aquaria. Additional studies are warranted on the role of the microbiome in the health of the developing lobster embryo and where the microbiome originates, if not from the surrounding seawater.

Indexed as

BacteriaEmbryo, NonmammalianMicrobiotaNephropidaeAnimalsFemaleLarvaRNA, Ribosomal, 16STemperatureRNA, Ribosomal, 16SClimate changeCrustaceaMetabarcodingMicrobiome

Identifiers

PMID41690962
PMCPMC12992824

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.