Evidence map›Paper›PMID 41690928›Full record

ArticleNature communications2026

Do-it-yourself protein arrays as high-throughput immunoassays enabled by confined droplets on patterned plasmonic chips.

Ying Yue, Chenyu Shi, Yichen Wang, Shengjie Ma, Bin Sun, Qiqi Kan, Zhengchao Ji, Shuai Dong, Xue Zheng, Hongli Shan and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Ying YueJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.
Chenyu ShiJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.
Yichen WangDepartment of Endocrinology, Lequn Branch, The First Hospital of Jilin University, Changchun, PR China.
Shengjie MaJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.
Bin SunJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.
Qiqi KanJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.
Zhengchao JiDepartment of Laboratory Medicine, The First Hospital of Jilin University, Changchun, PR China.
Shuai DongKey Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Department of Obstetrics, Obstetrics and Gynaecology Center, The First Hospital of Jilin University, Changchun, PR China.
Xue ZhengJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.
Hongli ShanDepartment of Laboratory Medicine, The First Hospital of Jilin University, Changchun, PR China.
Zheng HuKey Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Department of Obstetrics, Obstetrics and Gynaecology Center, The First Hospital of Jilin University, Changchun, PR China.ORCID http://orcid.org/0000-0001-8310-4224
Jiancheng XuDepartment of Laboratory Medicine, The First Hospital of Jilin University, Changchun, PR China.
Shoujun ZhuJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China. sjzhu@jlu.edu.cn.ORCID http://orcid.org/0000-0002-2412-6121
Junhu ZhangJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China. zjh@jlu.edu.cn.ORCID http://orcid.org/0000-0001-9100-6608
Bai YangJoint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, PR China.ORCID http://orcid.org/0000-0003-1939-6423

Funding

National Natural Science Foundation of China (National Science Foundation of China) Grant No. 22275071
6 · The paper itself

Abstract

Protein chips and arrays in parallel enable high-throughput protein detection in small-volume biological fluids, however, the limitations in sensitivity, dependence on expensive/bulky external equipment, and the need for specialized technical personnel have restricted their application in clinical and laboratory settings. Here, we present a droplet-based do-it-yourself (DBDIY) array on patterned plasmonic chips for high-throughput immunoassays, via confined droplets and plasmonic enhanced near-infrared (NIR) fluorescence. The assay allows equipment-free microarray fabrication and highly sensitive detection using only a pipette. It dynamically profiles 10 cytokines over 40 days in individual mice with just 6 µL of serum/blood per time point and achieves 100% sensitivity and specificity for C-reactive protein (CRP) in 1 nL of peripheral whole blood samples from 112 children. Scalable to high-throughput analysis of cell culture and serum, this accessible system simplifies workflows, extends utility in resource-limited settings, and offers a next-generation tool for longitudinal animal studies and clinical diagnostics.

Indexed as

High-Throughput Screening AssaysProtein Array AnalysisAnimalsC-Reactive ProteinCytokinesHumansImmunoassayMiceSensitivity and SpecificityC-Reactive ProteinCytokines

Identifiers

PMID41690928
PMCPMC13022438

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.