Evidence map›Paper›PMID 41690590›Full record

ArticleThe Journal of biological chemistry2026

Dynamic assessment of the allocation of copper to cytochrome c oxidase using size-exclusion chromatography (SEC) combined with inductively coupled plasma mass spectrometry (ICP-MS).

Dina Secic, Megan E Bischoff, Lucas Schmidt, Warunya Panmanee, Juechen Yang, Jarek Meller, Katherine E Vest, John T Cunningham, Julio A Landero, Maria F Czyzyk-Krzeska

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Dina SecicDepartment of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
Megan E BischoffDepartment of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
Lucas SchmidtDepartment of Environmental Medicine and Climate Science, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Warunya PanmaneeDepartment of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
Juechen YangDepartment of Biostatistics, Health Informatics and Data Sciences, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA; Division of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Jarek MellerDepartment of Biostatistics, Health Informatics and Data Sciences, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA; Division of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA; Institute of Engineering and Technology, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland.
Katherine E VestDepartment of Molecular and Cellular Biosciences, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
John T CunninghamDepartment of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
Julio A LanderoDepartment of Environmental Medicine and Climate Science, Icahn School of Medicine at Mount Sinai, New York, New York, USA. Electronic address: julio.landero@mssm.edu.
Maria F Czyzyk-KrzeskaDepartment of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA; Veteran Affairs Medical Center, Department of Veterans Affairs, Cincinnati, Ohio, USA; Department of Pharmacology, Physiology, and Neurobiology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. Electronic address: Maria.Czyzykkrzeska@uc.edu.

Funding

ENVIRONMETAL CARCINOGENESIS AND MUTAGENESIST32ES007250 · NIEHS · UNIVERSITY OF CINCINNATI · PI MILLER, WILLIAM E · 1988 to 2024
$11.9M
Function and regulation of copper in mammalian tissue differentiationR35GM146878 · NIGMS · UNIVERSITY OF CINCINNATI · PI Katherine Elizabeth Vest · 2022 to 2026
$2.4M
Metabolic effects of cooper in renal cancerR01CA287260 · NCI · UNIVERSITY OF CINCINNATI · PI Maria F Czyzyk-Krzeska · 2023 to 2026
$2.2M
BLRD VA I01 BX001110NCI NIH HHS R01 CA287260NIEHS NIH HHS T32 ES007250NIGMS NIH HHS R35 GM146878
6 · The paper itself

Abstract

Copper (Cu) is an essential trace element required for mitochondrial respiration via its incorporation into cytochrome c oxidase (CuCOX), the terminal enzyme of the electron transport chain. Here, we employed size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry (SEC-ICP-MS), UV-Vis spectroscopy, and immunoblotting to identify and validate a high molecular weight Cu-containing peak in the SEC-ICP-MS chromatogram as representative of CuCOX activity. We demonstrate that this CuCOX peak is enhanced under metabolic conditions inducing oxidative phosphorylation, such as high Cu supplementation or galactose-containing media, and correlates with increased mitochondrial respiration. Using exogenous

Indexed as

CopperElectron Transport Complex IVCell Line, TumorChromatography, GelHumansMass SpectrometryCopperElectron Transport Complex IVcoppercytochrome c oxidaserenal cancerSEC-ICP-MS

Identifiers

PMID41690590
PMCPMC12995813

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.