Evidence map›Paper›PMID 41690142›Full record

ArticlePoultry science2026

Development of an RT-ERA-CRISPR/Cas12a assay for duck Tembusu virus.

Zhanhong Huang, Yangbao Ding, Haocheng Xu, Qi Zhang, Xiyi Wang, Huamao Ding, Junsheng Zhang, Yufei Wang, Yingying Wang, Peirong Jiao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhanhong HuangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China; Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China.
Yangbao DingCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Technology Center of Nanning Customs, Nanning, Guangxi, China.
Haocheng XuCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Qi ZhangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Xiyi WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Huamao DingCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Junsheng ZhangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Yufei WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Yingying WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Peirong JiaoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China; Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China. Electronic address: prjiao@scau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck Tembusu virus (DTMUV) has continued to threaten the duck industry in China since 2010. Therefore, the establishment of a rapid, specific, and sensitive method for the field detection of DTMUV is urgently needed. Herein, a reverse transcription enzymatic recombinase amplification (RT-ERA) assay was combined with the CRISPR/Cas12a system to target the DTMUV C gene. This assay exhibited high specificity, effectively distinguishing DTMUV from other common avian viruses. Its limit of detection reached 1 copy/μL DTMUV RNA. Moreover, this assay can be completed at 42 °C within 15 min using a thermostatic water bath. Additionally, we tested 30 clinical samples from infected ducks using this assay, and the results showed 100% concordance with SYBR green quantitative PCR results. In summary, this rapid, specific, and sensitive assay shows promising potential for DTMUV detection in the field.

Indexed as

CRISPR-Cas SystemsDucksFlavivirusFlavivirus InfectionsNucleic Acid Amplification TechniquesPoultry DiseasesAnimalsChinaSensitivity and SpecificityCRISPR/Cas12aDetection methodsDuckRT‒ERATembusu virus

Identifiers

PMID41690142
PMCPMC12925148

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.