ArticleSTAR protocols2026
Spatiotemporal analysis of cell division during symbiotic root nodule development in the model legume Medicago truncatula.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Authors and funding
3 authors.
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Abstract
This protocol combines rhizobial spot inoculation with deep-tissue imaging to capture cellular processes during early nodule development in the legume Medicago truncatula. We describe steps to visualize DNA replication activity and cell geometry as indicators of cell proliferation and cell expansion processes. We detail steps for rhizobial spot inoculation of seedlings, incubation in 5-ethynyl-2'-deoxyuridine (EdU) medium, sample fixation and labeling of replicated DNA, clearing, staining of the cell walls, followed by confocal imaging. For complete details on the use and execution of this protocol, please refer to Lee et al.
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