Evidence map›Paper›PMID 41688649›Full record

ArticleScientific reports2026

Enzyme-mediated synergistic bioremediation of PAH and heavy metal co-contaminated soil using nocardia species and Helianthus annuus.

Akbar Ghasemi, Seyed Ali Abtahi, Mojtaba Jafarinia, Davood Azadi

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Akbar GhasemiDepartment of Soil Science, Marv.C., Islamic Azad University, Marvdasht, Iran.
Seyed Ali AbtahiDepartment of Soil Science, Marv.C., Islamic Azad University, Marvdasht, Iran.
Mojtaba JafariniaDepartment of Biology, Marv.C., Islamic Azad University, Marvdasht, Iran. mojtaba.jafarinia@iau.ac.ir.
Davood AzadiDepartment of Microbiology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Funding

Islamic azad University, Marvdasht branch 14020011
6 · The paper itself

Abstract

Heavy metals and polycyclic aromatic hydrocarbons (PAHs) are persistent soil contaminants posing risks to human health, environmental stability, and agricultural productivity via oxidative stress, neurotoxicity, carcinogenicity, and reduced crop yields. This study evaluated the bioremediation potential of native Iranian Nocardia species combined with sunflower (Helianthus annuus) remediation for restoring PAHs and heavy metal-co-contaminated soils. Thirty-six soil samples from Fars Province, Iran, were analyzed to isolate bioremediating Nocardia using turbidometric, chromatographic, biochemical, molecular, and enzymatic assays. Greenhouse experiments employed spiked soils (PAHs: 100-200 mg/kg; heavy metals: As, Hg, Cd, Pb, Cr; 3-400 mg/kg) across low, medium, and high levels, with five treatments (control, phytoremediation(P), bioremediation(B), combined phytoremediation and bioremediation (MB), sterile control) assessed over 90 days for soil properties, contaminants, plant growth, translocation (TF)/bioaccumulation factors (BAF). Thirteen Nocardia isolates (36.11%) were identified belonging to eight species. N. farcinica (85%), N. otitidiscaviarum (82%), and N. cyriacigeorgica (80%) led in PAH degradation; N. wallacei (75%), N. kruczakiae (70%), and N. cyriacigeorgica (65%) excelled in heavy metal removal. This results showed that the first report of identification of N. wallacei and N. veterana as PAHs degrading and heavy metals removal agents. A consortium of these five species in the MB treatment achieved 84.5-92.3% PAH degradation and 69.7-78.9% heavy metal removal, significantly outperforming B (76.8-85.6%, 63.4-72.5%), P (55.2-65.4%, 48.6-58.7%), and controls (p < 0.001). MB enhanced soil fertility (OC: 1.8%, TN: 42 mg/kg), microbial activity, and reduced metal translocation (TF/BAF: 0.20-0.60). In conclusion integrated Nocardia bioremediation and sunflower phytoremediation offers a promising sustainable strategy for co-contaminated soils, with potential for field-scale application in industrial and agricultural contexts following further validation.

Indexed as

Biodegradation, EnvironmentalHelianthusMetals, HeavyNocardiaPolycyclic Aromatic HydrocarbonsSoil PollutantsIranSoilSoil MicrobiologyMetals, HeavyPolycyclic Aromatic HydrocarbonsSoilSoil PollutantsBioremediationHeavy metalsNocardiaPAHsSoil remediationSunflower

Identifiers

PMID41688649
PMCPMC12982774

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.