Evidence map›Paper›PMID 41688524›Full record

ArticleScientific reports2026

Metabolomic assessment reveals depletion of amino acids and energy metabolites in skeletal muscle after ischemic acute kidney injury in mice.

Amy S Li, Peter R Baker, Samel Park, Isadore Budnick, Zhibin He, Kayo Okamura, Hyo-Wook Gil, Makoto Miyazaki, Colin C Anderson, Julie A Reisz and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Amy S Li *Division of Renal Diseases and Hypertension, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Box C281, Aurora, CO, 80045, USA.
Peter R Baker *Section of Clinical Genetics and Metabolism, Department of Pediatrics, University of Colorado Anschutz Medical Campus, 13123 East 16th Avenue, Box 300, Aurora, CO, 80045, USA.
Samel ParkDivision of Renal Diseases and Hypertension, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Box C281, Aurora, CO, 80045, USA.
Isadore BudnickDivision of Pulmonary Sciences and Critical Care Medicine, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Zhibin HeDivision of Renal Diseases and Hypertension, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Box C281, Aurora, CO, 80045, USA.
Kayo OkamuraDivision of Renal Diseases and Hypertension, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Box C281, Aurora, CO, 80045, USA.
Hyo-Wook GilDepartment of Internal Medicine, Soonchunhyang University Cheonan Hospital, Cheonan, Republic of Korea.
Makoto MiyazakiDivision of Renal Diseases and Hypertension, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Box C281, Aurora, CO, 80045, USA.
Colin C AndersonDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, 12801 East 17th Avenue, Aurora, CO, 80045, USA.
Julie A ReiszDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, 12801 East 17th Avenue, Aurora, CO, 80045, USA.
Sarah FaubelDivision of Renal Diseases and Hypertension, Department of Internal Medicine, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Box C281, Aurora, CO, 80045, USA. sarah.faubel@cuanschutz.edu.

Funding

PILOT STUDY--SUBSTRATE METABOLISM IN EXTREMELY LOW BIRTH WEIGHT INFANTSP30DK048520 · NIDDK · UNIVERSITY OF COLORADO DENVER · PI Wendy M Kohrt · 1995 to 2026
$32.6M
The role of MLKL in the regulation of vascular calcification in CKDR01HL132318 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI MIYAZAKI, MAKOTO · 2016 to 2025
$3.8M
The transcriptional control of vascular calcification in diseaseR01HL157064 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI Makoto Miyazaki · 2023 to 2026
$2.5M
Cardiac dysfunction after ischemic AKI in miceR01HL157973 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI FAUBEL, SARAH G · 2021 to 2024
$2.4M
A Novel Driver of Hyperphosphatemia and Vascular Calcification in CKDR01DK124901 · NIDDK · UNIVERSITY OF COLORADO DENVER · PI BLAINE, JUDITH T.,, MIYAZAKI, MAKOTO · 2021 to 2024
$1.9M
NHLBI NIH HHS R01 HL132318NHLBI NIH HHS R01 HL157064NHLBI NIH HHS R01 HL157973NIDDK NIH HHS P30 DK048520NIDDK NIH HHS R01 DK124901
6 · The paper itself

Abstract

Acute kidney injury (AKI) is a systemic catabolic condition that affects multiple organs. Clinically, AKI has been associated with muscle wasting, weakness, and delayed functional recovery. The underlying biochemical mechanisms driving these changes are not well understood. Ischemic AKI was induced in 8-10-week-old male C57BL/6 mice. Untargeted metabolomics on gastrocnemius samples were performed via ultra-high performance liquid chromatography-mass spectrometry at 24 h and 72 h after AKI. Of the 175 annotated analytes identified, 72 were significantly affected at 24 h with the majority of these metabolites being depleted in AKI compared to controls. Key depleted metabolites included multiple amino acids, glutathione and its precursors, and other energy-related substrates. Integration with our previously published metabolomics data in the kidney, liver, heart, and plasma highlights shared metabolic pathways across these organs, particularly reflected in arginine metabolism and the urea cycle, alanine/aspartate/glutamate metabolism, and glutathione/redox balance. This is the first study to characterize the metabolic profile of skeletal muscle after ischemic AKI in a murine model. Our data deepen the understanding of AKI as a systemic metabolic disease, underscoring the need to further understand the effects of AKI on skeletal muscle and opening potential avenues for therapeutic strategies to improve outcomes after AKI.

Indexed as

Acute Kidney InjuryAmino AcidsEnergy MetabolismIschemiaMetabolomeMetabolomicsMuscle, SkeletalAnimalsDisease Models, AnimalGlutathioneMaleMiceMice, Inbred C57BLAmino AcidsGlutathione

Identifiers

PMID41688524
PMCPMC12982615

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.