Evidence map›Paper›PMID 41686641›Full record

ArticleSTAR protocols2026

Protocol for intranuclear Nrf2 detection in activated mouse, human, and Jurkat T cells by spectral flow cytometry.

Aprajita Tripathi, Debolina Dasgupta, Mehak Ahuja, Gabrielle Fisette, Nadine Santana-Magal, Kalyani Pyaram

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Aprajita TripathiDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Debolina DasguptaDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Mehak AhujaDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Gabrielle FisetteDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Nadine Santana-MagalDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Kalyani PyaramDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA. Electronic address: kpyaram@kumc.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nuclear factor erythroid 2-related factor 2 (Nrf2) coordinates cellular antioxidation responses. Its expression increases in T cells upon activation. Here, we present a protocol for quantitative detection of intranuclear Nrf2 in activated mouse, human, and Jurkat T cells using spectral flow cytometry. We describe procedures for primary T cell isolation, in vitro activation, intranuclear staining, and flow cytometric acquisition and analysis, including gating strategies for CD4

Indexed as

Cell NucleusFlow CytometryNF-E2-Related Factor 2T-LymphocytesAnimalsHumansJurkat CellsLymphocyte ActivationMiceNF-E2-Related Factor 2AntibodyCell-based AssaysCell cultureFlow CytometryImmunology

Identifiers

PMID41686641
PMCPMC12915187

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.