ArticleJournal of assisted reproduction and genetics2026
Validation and clinical study of single-step vitrification combined with single-step warming of human blastocysts.
Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07556601 (Clinical Validation of Ultra-rapid Blastocyst Vitrification), which is not on this map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Clinical Validation of Ultra-rapid Blastocyst Vitrification: a Randomized Pilot Study in Oocyte Donation Cycles
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis study assesses the feasibility of single-step vitrification and warming (SSV/SSW) for human blastocysts. Previous animal study models have shown possibilities of shorter cryopreservation methodologies by cryoprotectant and temperature adjustments. The main research question investigates whether SSV/SSW can serve as an effective alternative to stepwise vitrification (SWV) and warming procedures, ensuring comparable embryological and clinical outcomes.
methodThe study was conducted at a single center. Validation of SSV combined with SSW assessing survival, re-expansion, and development after 24 h was performed using research blastocysts. Cryoprotectant (CPA) levels were measured in three different SSW sucrose solutions after SSV or SWV. We introduced the valid method in the clinic and report laboratory and clinical outcomes of 333 SWV/SSW against 568 SSV/SSW blastocyst transfers.
resultsDuring validation, SSV at 37 °C, compared to SWV, combined with 1 M sucrose SSW demonstrated comparable collapsing (89.6% vs. 98.1%) and re-expansion outcomes (97.9% vs. 88.7%). Extracellular CPA analysis (79.3 mg/L vs. 127.3 mg/L; p < 0.001) indicated significantly lower intracellular uptake following SSV compared to SWV. Clinical data showed significantly lower rates of collapsing (67.8% vs. 92.5%, p < 0.001) and re-expansion (72.0% vs. 89.5%, p < 0.001) after SSV compared to SWV, but similar rates of ongoing pregnancy (25.9% vs. 26.1%) and secondary clinical outcomes.
conclusionSSV combined with SSW is a feasible, operationally efficient alternative to SWV and warming procedures open to further optimize the collapse status before or during vitrification. The method reduces handling time, CPA exposure, and operator-related errors while maintaining ongoing pregnancy rates.
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