Evidence map›Paper›PMID 41686287›Full record

ArticleRice (New York, N.Y.)2026

Impairment of the Uroporphyrinogen Decarboxylase 2 Gene Induces Lesion Mimicry and Downregulates Tetrapyrrole Biosynthesis Pathway Genes in Rice.

Mengyu Qu, Yan Lin, Rong Lin, Donghai Song, Haomin Zhang, Fujia Yang, Ziqiang Chen, Weiqi Tang, Yijuan Han, Wenqing Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Rice (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mengyu QuState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yan LinBiotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.
Rong LinMarine and Agricultural Biotechnology Laboratory, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China.
Donghai SongState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Haomin ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Fujia YangMarine and Agricultural Biotechnology Laboratory, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China.
Ziqiang ChenBiotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.
Weiqi TangMarine and Agricultural Biotechnology Laboratory, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China.
Yijuan HanMarine and Agricultural Biotechnology Laboratory, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China.
Wenqing LiTobacco-Rice Synergistic Development Industrial Technology Engineering Center, State Tobacco Monopoly Administration (China National Tobacco Corporation), Fuzhou, 350013, China.
Zonghua WangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Guodong LuState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. lgd@fafu.edu.cn.
Songbiao ChenMarine and Agricultural Biotechnology Laboratory, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China. sbchen@fjage.org.

Funding

National Natural Science Foundation of China 32071941National Natural Science Foundation of China U2005211Natural Science Foundation of Fujian Province 2023J011571
6 · The paper itself

Abstract

Lesion-mimic mutants (LMMs) spontaneously develop necrotic lesions in the absence of pathogen infection or environmental stress, serving as ideal models for studying cell death, immune signaling, and the growth-defense balance in plants. In this study, we identified a rice LMM and mapped the causal mutation to the Uroporphyrinogen Decarboxylase 2 gene, which had not been previously functionally characterized within the tetrapyrrole biosynthesis (TBS) pathway. Knockdown of OsUROD2 resulted in lesion mimicry and down-regulated key TBS pathway genes, including OsCPOX, OsPPOX, OsChlH, OsChlD, OsChlI, and OsChlM. Further functional analyses revealed that OsCPOX knockdown or OsPPOX knockout both caused lesion formation and reduced chlorophyll content, whereas triple knockout of OsChlH/OsChlD/OsChlI or knockout of OsChlM resulted in chlorophyll deficiency. Subcellular localization assays showed that OsUROD2, along with OsCPOX, OsPPOX, OsChlH, OsChlD, OsChlI, and OsChlM, localizes to the chloroplast. Our results demonstrate that the chloroplast-localized TBS pathway is integral to viability, lesion mimicry and chlorophyll synthesis in rice, providing crucial insights into the molecular mechanisms linking tetrapyrrole metabolism to regulated cell death.

Indexed as

Cell deathChlorophyll synthesisLesion mimicryOsUROD2RiceTetrapyrrole biosynthesis

Identifiers

PMID41686287
PMCPMC13004804

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.