ArticleJournal of experimental botany2026
A pennycress transparent testa 8 knockout mutant has drastic changes in seed coat anatomy and chemical compositions.
Article in Journal of experimental botany, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Seed biology: the birth of plant life.Journal of experimental botany · 2026Article
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10 authors.
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Abstract
Pennycress is a winter annual intermediate crop with ∼30% seed oil content suitable for producing biofuels. Here, we evaluated seed development, anatomy, and agronomically relevant traits of a transparent testa 8 knockout mutant (tt8-2bp) generated by CRISPR genome editing to improve seed quality. We performed histochemical analyses on wild-type and tt8-2bp seeds at different developmental stages. No visible anatomical defects were observed in embryos and endosperm of tt8-2bp seeds. However, tt8-2bp seed coats had drastically reduced proanthocyanidins and proanthocyanidin monomers, which correlated with increased seed coat permeability, increased imbibition rates, and altered seed aging of tt8-2bp seeds. A cuticle layer was detected in tt8-2bp and wild-type seed coats. Further analysis is required to assess possible quantitative and structural defects in the tt8-2bp seed cuticle. Based on metabolomic and solid-state NMR analyses, tt8-2bp seed coats had decreased aromatic compounds and cell wall polysaccharides compared with wild-type seed coats. Consistently, tt8-2bp seeds also had reduced non-embryonic tissue dry weights, increased embryo dry weights, and unchanged total seed weights compared with wild-type seeds. This indicated altered nutrient partitioning during tt8-2bp seed development. The agronomic implications of tt8-2bp altered seed traits on pennycress domestication are discussed in depth.
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