ArticleEuropean endodontic journal2025
Antimicrobial Efficacy of Calcium Hydroxide Nanoparticles Combined with Nisin Against Enterococcus Faecalis in Root Canal Infections ��� an Ex Vivo Study.
Article in European endodontic journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The trial behind it
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Who cites it
2 citing papers in PubMed.
- Antimicrobial effectiveness of intracanal medicaments againstFrontiers in oral health · 2026Review
- Assessment of Sealing Ability and Degradation Resistance of a Hydrogel-Based Root Canal Filling Material Using a Bacterial Leakage Model and SEM Analysis.BioMed research international · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
objectiveThis ex-vivo study aimed to evaluate the antimicrobial effects of calcium hydroxide (Ca(OH)2) nanoparticles combined with nisin against Enterococcus faecalis in infected root canals.
methodsThe Minimum Inhibitory Concentration (MIC50) and Fractional Inhibitory Concentration (FIC) index were determined to assess the potential synergistic effect of the combination. Root canal specimens infected with E. faecalis were divided into three treatment groups: Ca(OH)2 nanoparticles with nisin, Ca(OH)2 nanoparticles alone, and a control group with no treatment. Colony Forming Units (CFU) were quantified at different dentinal depths, and the relative gene expression of E. faecalis was analysed using quantitative PCR.
resultsMIC50 was not achieved by Ca(OH)2, while nisin had an MIC of 50 mg/mL. FIC analysis indicated no synergistic interaction between Ca(OH)2 and nisin. From the root canal specimen model, Ca(OH)2 nanoparticles alone and the combination of Ca(OH)2 nanoparticles and nisin disinfected dentinal tubules up to 1.3 mm. However, neither group achieved disinfection at a depth of 1.5 mm. Relative gene expression analysis showed that the combination treatment significantly reduced E. faecalis gene expression compared to Ca(OH)2 alone.
conclusionThe combination of Ca(OH)2 nanoparticles and nisin does not improve the antimicrobial activity of Ca(OH)2 nanoparticles against E. faecalis, suggesting independent antimicrobial activity of both agents. (EEJ-2025-04-054).
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