Decellularized Nerve Scaffold in a Rat Model of Extensive Peripheral Nerve Damage.

T V Rusinova, R A Vinogradov, A S Asyakina, G P Chuprynin, A A Fomenko, E A Solop, K I Melkonyan

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Article in Sovremennye tekhnologii v meditsine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

T V RusinovaPhD, Researcher, Central Research Laboratory; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

R A VinogradovMD, DSc, Associate Professor, Professor, Department of Surgery No.1; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

A S AsyakinaJunior Researcher, Central Research Laboratory; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

G P ChupryninResearch Laboratory Assistant, Central Research Laboratory; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

A A FomenkoResearch Laboratory Assistant, Central Research Laboratory; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

E A SolopResearch Laboratory Assistant, Central Research Laboratory; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

K I MelkonyanMD, PhD, Associate Professor, Head of Central Research Laboratory; Kuban State Medical University, 4 Mitrofana Sedina St., Krasnodar, 350063, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Materials and Methods: The study described a modified detergent-enzymatic decellularization protocol to create a decellularized nerve scaffold. A decellularization process (24 h) included the sequential treatment of a rat sciatic nerve with the solutions of trypsin-versene, 1% Triton X-100, 4% sodium deoxycholate, phosphate-buffered saline, and pancreatic DNase I. We modelled an extended defect of the sciatic nerve (15±2 mm), and Wistar rats were implanted with autografts or decellularized nerve scaffolds. The nerve recovery was assessed on day 90 after implantation using an immunohistochemistry analysis of the total count of nerve fibers, intact motor fibers, and myelinated fibers. Results: The results of the histological examination and DAPI staining showed the complete destruction and washing of nuclear material to preserve the nerve structure after decellularization. The DNA content in the decellularized scaffolds was 48.17±4.25 ng/mg of tissue, while in the native nerve it was 221.51±1.36 ng/mg. The analysis of the tissue response to the decellularized scaffold subcutaneously implanted demonstrated the absence of macrophages. Histology revealed a moderate number of intact nerve fibers in the orthotopically implanted decellularized scaffold (835.6 [804.2; 866.0] per 1 mm Conclusion: The use of detergent-enzymatic decellularization of the rat sciatic nerve demonstrated high efficacy, which was confirmed by the absence of nuclear material with the nerve histological structure preserved. The presence of the sufficient number of Schwann cells 3 months after implantation and the perineurium formation are the positive characteristics when assessing the nerve decellularization protocol efficacy. Thus, the decellularized nerve scaffold is a promising replacement of autografts to treat extensive defects of peripheral nerves.

Indexed as

Decellularized Extracellular MatrixNerve RegenerationPeripheral Nerve InjuriesSciatic NerveTissue ScaffoldsAnimalsDisease Models, AnimalMaleRatsRats, WistarTissue EngineeringDecellularized Extracellular Matrixautograftdecellularizationdecellularized matrixnerve scaffoldperipheral nerve regeneration

Identifiers

PMID41684871

DOI10.17691/stm2025.17.6.02

PMCPMC12892847

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