Mild One-Step Protein Recovery from Microalgae Cultivated in Swine Wastewater Using Natural Deep Eutectic Solvent-Based Aqueous Biphasic Systems.

David Moldes, Marisol Vega, Silvia Bolado, Patricia F Requejo

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

David MoldesInstitute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain.ORCID 0000-0002-9904-3056

Marisol VegaInstitute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain.ORCID 0000-0002-3635-7920

Silvia BoladoInstitute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain.ORCID 0000-0003-0941-3306

Patricia F RequejoInstitute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Photobioreactor-based microalgae cultivation offers an integrated approach for nutrient-rich wastewater treatment while producing valuable biomass. One of the main microalgae components is proteins, making them a biotechnological target. In this work, to develop efficient and greener extraction methodologies, aqueous two-phase systems (ATPSs) based on natural deep eutectic solvents (NADESs) were evaluated for one-step protein extraction from microalgae cultivated in swine wastewater. Six ATPSs combining two NADES-betaine:levulinic acid (Bet:2LA) and choline chloride:urea (ChCl:2Urea)-and their individual components (Bet or ChCl) with phosphate salts were compared. Systems {NADES + K

Indexed as

Deep Eutectic SolventsMicroalgaeWastewaterAnimalsBetaineBiomassCholinePhotobioreactorsSolventsSwineWaterBetaineCholineDeep Eutectic SolventsSolventsWastewaterWateraqueous two-phase system (ATPS)green extractionprotein recovery

Identifiers

PMID41683462

DOI10.3390/molecules31030483

PMCPMC12899697

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.