ArticleFoods (Basel, Switzerland)2026
A Chemiluminescent Magnetic Enzyme Immunoassay Method for 2 Triazole Pesticide Detection in Wheat.
Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
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9 authors.
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Abstract
We developed an alkaline phosphatase (AP) chemiluminescence immunoassay method by combining the superparamagnetic magnetic beads and the biotin-streptavidin signal amplification system to detect the triazolone and tebuconazole in wheat. Through optimization of the extraction solution and extraction time, acetonitrile-PBS was selected as the extraction solution with an extraction time of 5 min as the optimal pretreatment condition. Optimizing the dilution ratio of antigen antibodies, the optimal detection conditions were selected as the dilution ratios of 1:8000 and 1:20,000 for the triazolone monoclonal antibody solution and biotinylated triazolone solution, and 1:4000 and 1:20,000 for the tebuconazole monoclonal antibody solution and biotinylated tebuconazole solution, respectively. Under the optimal conditions, the method demonstrated that the limits of detection (LOD) of triazolone and tebuconazole were 0.002835 μg·mL
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