ArticleFoods (Basel, Switzerland)2026
Structural Characterization and Emulsifying Properties of Highly Soluble Macadamia-Soybean Protein Composites Fabricated by Alkaline-Thermal Treatment.
Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The complementarity of plant proteins from various sources could achieve higher nutritional value to satisfy the requirement of replacing animal proteins. Therefore, it is very important to seek efficient and convenient approaches to fabricate highly soluble protein composites. In this study, macadamia protein-soybean protein (SP-MP') composites were fabricated by alkaline-thermal treating at different ratios of 1:0.5, 1:1, and 1:2; then, the nitrogen solubility index, particle characteristics, and structure and emulsifying properties of SP-MP' composites were investigated. The nitrogen solubility indexes of SP-MP' composites were higher than 80%, and less small insoluble aggregates were observed by scanning electron microscopy. SP-MP' composites exhibited high ζ-potential values, which were higher than -50 mV. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis found that both subunits of individually alkaline-thermal-treated macadamia protein (MP') and soybean protein (SP') were presented in SP-MP' composites. The results of fluorescence, sulfhydryl group, and secondary structure illustrated that the SP interacted with MP to form SP-MP' composites by the co-folding of proteins during neutralization. Compared to the individual proteins, SP-MP' composites exhibited stronger emulsification ability and stability indexes (EAI and ESI) as the proportion of MP increased, and the EAI and ESI of SP-MP
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