Evidence map›Paper›PMID 41681529›Full record

ReviewPlants (Basel, Switzerland)2026

Nanoparticle Applications in Plant Biotechnology: A Comprehensive Review.

Viktor Husak, Milos Faltus, Alois Bilavcik, Stanislav Narozhnyi, Olena Bobrova

Abstract readReview
In one paragraph

Review in Plants (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Viktor HusakDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Carpathian National University, Shevchenka 57, 76018 Ivano-Frankivsk, Ukraine.ORCID 0000-0001-9415-9837
Milos FaltusCzech Agrifood Research Center, Plant Physiology and Cryobiology Team, Drnovska 507/73, 16100 Prague, Czech Republic.ORCID 0000-0003-2217-5226
Alois BilavcikCzech Agrifood Research Center, Plant Physiology and Cryobiology Team, Drnovska 507/73, 16100 Prague, Czech Republic.ORCID 0000-0002-8588-2924
Stanislav NarozhnyiCryobiophysics Department, Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine, Pereyaslavskaya 23, 61016 Kharkiv, Ukraine.
Olena BobrovaCzech Agrifood Research Center, Plant Physiology and Cryobiology Team, Drnovska 507/73, 16100 Prague, Czech Republic.ORCID 0000-0001-9100-1823

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanotechnology is becoming a key tool in plant biotechnology, enabling nanoparticles (NPs) to deliver biomolecules with high precision and to enhance plant and tissue resilience under stress. However, the literature remains fragmented across genetic delivery, in vitro regeneration, stress mitigation, and germplasm cryopreservation, and it still lacks standardized, comparable protocols and robust long-term safety assessments-particularly for NP use in cryogenic workflows. This review critically integrates recent advances in NP-enabled (i) genetic engineering and transformation, (ii) tissue culture and regeneration, (iii) nanofertilization and abiotic stress mitigation, and (iv) cryopreservation of plant germplasm. Across these areas, the most consistent findings indicate that NPs can facilitate targeted transport of DNA, RNA, proteins, and regulatory complexes; modulate oxidative and osmotic stress responses; and improve regeneration performance in recalcitrant species. In cryopreservation, selected nanomaterials act as multifunctional cryoprotective adjuvants by suppressing oxidative injury, stabilizing cellular membranes, and improving post-thaw viability and regrowth of sensitive tissues. At the same time, NP outcomes are highly context-dependent, with efficacy governed by dose, size, and surface chemistry; formulation; plant genotype; and interactions with culture media or vitrification solutions. Evidence of potential phytotoxicity, persistence, and biosafety risks highlights the need for harmonized reporting, mechanistic studies on NP-cell interfaces, and evaluation of environmental fate. Expected outcomes of this review include a consolidated framework linking NP properties to biological endpoints, identification of design principles for application-specific NP selection, and a set of research priorities to accelerate the safe and reproducible translation of nanotechnology into sustainable plant biotechnology and long-term germplasm preservation.

Indexed as

biocompatibilitycryopreservationgenetic transformationgreen synthesisoxidative stresstissue culture

Identifiers

PMID41681529
PMCPMC12899883

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.