Evidence map›Paper›PMID 41680160›Full record

ArticleNature communications2026

PRICE: direct and robust detection of microRNAs at single-nucleotide resolution.

Buhua Wang, Shuai Zhou, Xi Zhang, Rui Wang, Shuo Huang, Anyi Li, Haowei He, Yingai Zhang, Yangdao Wei, Zhiqing Yang and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Buhua Wang *School of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.ORCID 0000-0002-7026-6360
Shuai Zhou *Department of Hepatobiliary Surgery, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China.
Xi ZhangSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.
Rui WangNHC Key Laboratory of Tropical Disease Control, Hainan Medical University, Haikou, China.
Shuo HuangDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Anyi LiSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.
Haowei HeCentral Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou City Key Laboratory of Clinical Medicine, Haikou, China.
Yingai ZhangCentral Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou City Key Laboratory of Clinical Medicine, Haikou, China.
Yangdao WeiDepartment of Clinical Hematology and Institute of Materia Medica, College of Pharmacy and Laboratory Medicine Science, Army Medical University, Chongqing, China.
Zhiqing YangSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.
Fengge SongSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.ORCID 0000-0003-2296-4225
Xiangpeng LiDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA.
Xinyi WanSchool of Environmental Science and Engineering, Hainan University, Haikou, China.
Yi ShenSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.
Chunxin MaSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China.
Haimei MaoProducts Quality Supervision and Testing Institute of Hainan Province, Haikou, China.
Rodrigo Ledesma-AmaroDepartment of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.ORCID 0000-0003-2631-5898
Dapeng YinHainan Center for Disease Control and Prevention, Haikou, China.
Qingshan WeiDepartment of Chemical and Biomolecular Engineering, Emerging Plant Disease and Global Food Security Cluster, North Carolina State University, Raleigh, NC, USA.ORCID 0000-0003-1214-010X
Ruijie DengCollege of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China.ORCID 0000-0001-9094-124X
Tao YangDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Shanrong LiuDepartment of Laboratory Diagnostics, Changhai Hospital, Navy Military Medical University, Shanghai, China.
Yi WanSchool of Marine Sciences (State Key Laboratory of Marine Resource Utilization in South China Sea), Hainan University, Haikou, China. 993602@hainanu.edu.cn.ORCID 0000-0002-0667-3279
Chuanbin MaoDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China. cmao@cuhk.edu.hk.ORCID 0000-0002-8142-3659

Funding

Research Grants Council, University Grants Committee (RGC, UGC) 14208723
6 · The paper itself

Abstract

Accurate single-nucleotide discrimination of miRNA is clinically vital because small sequence variations can have significant phenotypic and clinical consequences, yet existing techniques can only detect single nucleotide variations (SNVs) at specific loci. Here, we present a generalized peptide nucleic acid (PNA) mediated CRISPR/Cas13a system (PRICE), enabling detection of SNVs in miRNA sequence without sacrificing the sensitivity. PRICE utilizes PNA blockers fully complementary to non-target miRNAs (e.g., miRNAs containing SNVs at loci of no interest) but not to the target miRNA. These blockers selectively hybridize with and inhibit non-target sequences in samples (serum, cells, or tissues). Only the unhybridized target miRNA then binds to crRNA within the Cas13a complex, activating Cas13a to cleave a fluorescent reporter-quencher linker, generating a detectable signal (~10 fM limit). By designing a panel of PNAs against SNVs, PRICE provides a versatile, amplification-free platform for precise miRNA analysis, advancing cancer diagnosis, prognosis, and biology.

Indexed as

CRISPR-Cas SystemsMicroRNAsPeptide Nucleic AcidsPolymorphism, Single NucleotideHumansMicroRNAsPeptide Nucleic Acids

Identifiers

PMID41680160
PMCPMC13004955

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.