ArticleAnimal bioscience2026
Effects of different dietary methionine and cysteine ratios on growth performance and intestinal development of broilers from brain-gut peptide secretion perspective.
Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
objectiveThe objective of this study is to investigate the nutritional metabolism and growth development of broiler chickens under different methionine (Met)-to-cysteine (Cys) ratios, with a focus on proteomic alterations in the hypothalamus and ileum to elucidate potential regulatory mechanisms.
methodsA total of 216 one-day-old Arbor Acres broiler chicks were randomized into three groups: low (LMCR, 0.34%:0.56%), medium (MMCR, 0.45%:0.45%), and high (HMCR, 0.53%:0.37%) Met-to-Cys ratios, with total sulfur amino acids fixed at 0.9%. Each group comprised 6 replicates of 12 chicks, and the experiment spanned 21 days. On day 22, the birds were fasted for 12 hours before slaughter to collect serum, hypothalamus and ileum samples for comprehensive analysis of metabolic hormones, neurotransmitters and proteomic profiles.
resultsResults demonstrated that HMCR improved average daily gain (p<0.05), reduced feed conversion ratio (p<0.05), and enhanced protein/lipid utilization efficiency. Mechanistically, HMCR promoted hypothalamic neurodevelopment, downregulated key components of the protein kinase A signaling pathway (p<0.05) and leptin expression (p<0.001), while upregulating growth hormone and gastrointestinal hormone secretion. Through the hypothalamic-hormonal-ileal axis, HMCR modulated ileal cell physiology by upregulating inositol 1,4,5-trisphosphate receptors (p<0.05) and phosphoinositide 3-kinase (p<0.05), thereby enhancing ileal villus morphology and nutrient absorption capacity. The improved ileal structure further augmented energy utilization efficiency (p<0.05).
conclusionIn conclusion, a dietary Met:Cys of 0.53%:0.37% optimizes broiler growth performance by coordinately regulating metabolic pathways in the hypothalamus and ileum, as well as modulating brain-gut peptide signaling. These findings provide a scientific foundation for formulating sulfur amino acid-optimized diets to enhance poultry productivity and feed efficiency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.