ArticleiMetaOmics2025
HiFi based metagenomic assembly strategy provides accuracy near isolated genome resolution in MAG assembly.
Article in iMetaOmics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Genome-resolved and culture-based atlas of the feline gut microbiome enables host-adapted probiotic development.NPJ biofilms and microbiomes · 2026Article
- 16S rRNA Gene and Metagenomic Analysis Revealed an Association Between Cecal Microbiota and Pork Umami.Animals : an open access journal from MDPI · 2026Article
- Whole-Genome Resequencing Reveals Population Structure and Loci Associated with Growth and Meat-Quality Traits in Meigu Yanying Chickens.Animals : an open access journal from MDPI · 2026Article
- Microoxic conditions promote Escherichia-associated cellulase expression in the giant panda gut.The ISME journal · 2026Article
- Holistic genome assembly and analysis of theIMA fungus · 2026Article
- Strain-resolved biology ofFrontiers in veterinary science · 2026Review
- High-Altitude Extreme Environments Drive Convergent Evolution of Skin Microbiota in Humans and Horses.Microorganisms · 2025Article
- Functional and Compositional Changes in Ileal Microbiota in Piglets During the Nursing Period Revealed by 16s rRNA Gene and Metagenomics.Animals : an open access journal from MDPI · 2025Article
- Gut Microbial Signatures of Broiler Lines Divergently Selected for Inosine Monophosphate and Intramuscular Fat Content.Animals : an open access journal from MDPI · 2025Article
- Bacterial-Fungal Interactions: Mutualism, Antagonism, and Competition.Life (Basel, Switzerland) · 2025Review
- Article
- Editorial: Advancements in synthetic microbiomes for enhancing animal health.Frontiers in veterinary science · 2025Article
- PartialFrontiers in veterinary science · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recovering high-contiguity, circular bacterial genomes from complex microbiomes (e.g., gut) is challenged by limitations of short-read and error-prone long-read sequencing. This study comprehensively compares PacBio High-Fidelity (HiFi) sequencing-based metagenome-assembled genomes (MAGs) against Illumina MAGs, Oxford Nanopore Technologies (ONT) MAGs, and isolate whole-genome sequencing genomes from the same sample. HiFi sequencing yielded 31 high-quality MAGs, including 10 complete circular genomes. HiFi MAGs demonstrated significantly higher completeness, continuity, and lower contamination than Illumina or ONT MAGs (
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.