AistSeq: An in-house easy-to-purify Tn5-based plasmid sequencing platform using a compact benchtop sequencer.

Hayato Suzuki, Jutapat Romsuk, Akiyoshi Nakamura, Kosuke Moriwaki, Yoko Yamanishi, Shigeo S Sugano

Abstract read

In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hayato Suzuki *Plant Molecular Production Research Team, Biomanufacturing Process Research Center, The National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan.

Jutapat Romsuk *Plant Functional Regulation Research Team, Biomanufacturing Process Research Center, The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Akiyoshi NakamuraPlant Functional Regulation Research Team, Biomanufacturing Process Research Center, The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Kosuke MoriwakiPlant Functional Regulation Research Team, Biomanufacturing Process Research Center, The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Yoko YamanishiDepartment of Mechanical Engineering, Kyushu University, Fukuoka, Japan.

Shigeo S SuganoPlant Functional Regulation Research Team, Biomanufacturing Process Research Center, The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sequence verification of plasmids is a fundamental process in synthetic biology. For plasmid sequence verification using next-generation sequencing (NGS) library preparation, Tn5 transposase is widely used. Streamlined sequencing workflow for laboratory-scale applications is important; however, recombinant Tn5 production in-house can be laborious. In this study, we demonstrated that the addition of a large soluble tag was not essential for purification and that the fusion of a His10 tag and protein A was sufficient to yield active Tn5 transposase in adequate amounts. In addition, we evaluated exonuclease-based genomic DNA digestion for plasmid sequencing from an

Indexed as

bench top sequencernext-generation sequencing (NGS)pipelineplasmid sequencingTn5 transposase purification

Identifiers

PMID41676073

DOI10.3389/fbioe.2025.1673510

PMCPMC12886386

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.