Evidence map›Paper›PMID 41675992›Full record

ArticleBio-protocol2026

Detailed Method for the Purification of Rhamnogalacturonan-I (RG-I) in

Liang Zhang, Tasleem Javaid, Breeanna R Urbanowicz

Abstract read
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Liang ZhangComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Tasleem JavaidComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Breeanna R UrbanowiczComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The plant cell wall is a dynamic and complex extracellular matrix that not only provides structural integrity and determines cell shape but also mediates intercellular communication. Among its major components, pectins play essential roles in cell adhesion, wall porosity, hydration, and flexibility. Rhamnogalacturonan-I (RG-I), a structurally diverse pectic polysaccharide, remains one of the least understood components of the plant cell wall. Its backbone is substituted with arabinan, galactan, and arabinogalactan side chains that vary in length, branching, and composition across tissues, species, and developmental stages. In addition, RG-I can undergo modifications such as backbone acetylation, further contributing to its structural complexity and functional diversity. To advance understanding of RG-I, we present a detailed method for isolating RG-I from the model plant

Indexed as

Alcohol-insoluble residue (AIR)Ammonium oxalateArabidopsis thalianaEndo-polygalacturonase (EPG)PectinPlant cell wallRhamnogalacturonan-I (RG-I)Size exclusion chromatography (SEC)

Identifiers

PMID41675992
PMCPMC12887878

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.