Evidence map›Paper›PMID 41675991›Full record

ArticleBio-protocol2026

A One-Step Method for Efficient Purification of Functional Cas9 Protein.

Xinzhi Duan, Zhengrong Zhou, Aihua Mao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Xinzhi DuanBiology Department, College of Sciences, Shantou University, Shantou, China.
Zhengrong ZhouNeuroscience center, Department of Basic Medical Sciences, Shantou University Medical College, Shantou, China.
Aihua MaoBiology Department, College of Sciences, Shantou University, Shantou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The CRISPR/Cas9 system is a cornerstone technology in genome editing. Delivery of pre-assembled Cas9 ribonucleoprotein (RNP) complexes exhibits distinct advantages, including reduced off-target effects and lower immunogenicity. Conventional methods for purifying Cas9 protein typically involve multi-step chromatography and the cleavage of fusion tag, which are time-consuming and result in diminished yields. In this study, we present a simplified, one-step purification strategy for functional

Indexed as

Affinity chromatographyGene editingProtein purificationSpCas9Ubiquitin-fusion

Identifiers

PMID41675991
PMCPMC12887862

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.