Evidence map›Paper›PMID 41675988›Full record

ArticleBio-protocol2026

Turbo-RIP: A Protocol for TurboID-based RNA Immunopurification to Map RNA Landscapes in Plant Biomolecular Condensates.

Zhi Zhang, Yanting Xu, Hanxiang Liu, Chen Liu, Panagiotis Nikolaou Moschou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhi ZhangState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Stress Biology, Innovation center for evolutionary synthetic biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Yanting XuState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Stress Biology, Innovation center for evolutionary synthetic biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Hanxiang LiuState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Stress Biology, Innovation center for evolutionary synthetic biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Chen LiuState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Stress Biology, Innovation center for evolutionary synthetic biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Panagiotis Nikolaou MoschouDepartment of Biology, University of Crete, Heraklion, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biomolecular condensates organize cellular processes through liquid-liquid phase separation, creating membrane-less compartments enriched in specific proteins and RNAs. Understanding their RNA composition is essential for elucidating plant stress responses, yet capturing these transiently associated RNAs remains technically challenging. We present Turbo-RIP (TurboID-based proximity labeling with RNA immunopurification), a comprehensive protocol for identifying condensate-associated RNAs in plants. Turbo-RIP employs the biotin ligase TurboID to label proximal proteins at 22 °C, followed by formaldehyde crosslinking and streptavidin-based capture of protein-RNA complexes. We provide detailed procedures for three cloning strategies, transformation of

Indexed as

Biomolecular condensatesLiquid–liquid phase separationPlant stress responsesProximity biotinylationRNA immunopurificationTurboID

Identifiers

PMID41675988
PMCPMC12887874

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.