ArticleFrontiers in molecular biosciences2026
A validated LC-ESI-MS/MS assay for simultaneous plasma quantification of 11 antimicrobials to support personalized dosing in critically ill children.
Article in Frontiers in molecular biosciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Critically ill patients with severe infections demonstrate profound alterations in pharmacokinetic behavior. Therapeutic drug monitoring (TDM)-informed antimicrobial dose optimization is thus essential in intensive care settings to ensure maximal bactericidal activity while mitigating toxicity, creating an urgent need for accessible analytical methodologies. Although various studies exist in this domain, the inherent technical constraints of current methodologies persistently prevent the full resolution of these unmet clinical requirements. Methods: A rapid and sensitive LC-ESI-MS/MS method was developed and validated for the simultaneous quantification of 11 antimicrobials in human plasma. Sample preparation was performed by a simple one-step protein precipitation using methanol containing 0.1% formic acid. The analytes were separated on a Kinetex C18 column with a "corner-folded cleaver-shaped" gradient elution program and detected using multiple reaction monitoring (MRM) in positive ionization mode. Results and Discussion: The gradient elution program achieved baseline separation of all tested analytes within 8 min, with symmetrical peak shapes and no endogenous interference. The developed method was proven to be free of matrix effects, excellent linearity (
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