ReviewiMetaOmics2024
Long journey of 16S rRNA-amplicon sequencing toward cell-based functional bacterial microbiota characterization.
Review in iMetaOmics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Bacterial metagenomic analysis of patients with chronic kidney disease undergoing hemodialysis based on 16S rDNA amplicon sequencing.Biomedical reports · 2026Article
- Innovative technologies and future perspectives in tumor microbiomics.Science China. Life sciences · 2026Review
- Marine influence on airborne bacterial community composition and predicted functional potential in coastal zones: a case study from Ostend, Belgium.Scientific reports · 2026Article
- Phytomicrobiome of Helianthus annuus: in vitro assessment of plant growth-promoting traits and heavy metal tolerance.BMC plant biology · 2026Article
- Effects of Exposure to Extreme Artificial Light at Night on Liver Oxidative Damage and Gut Microbiota During Pregnancy and Lactation in Mice.Animals : an open access journal from MDPI · 2026Article
- Accu16S/AccuITS: Accurate and broadly applicable amplicon sequencing for absolute microbiome quantification.iMeta · 2026Article
- Standardized and batch effect-independent technologies enable global collaboration in microbiome research.The ISME journal · 2026Article
- The effect of different milk pretreatment methods on microbiome community development during Herrgårds cheese production and ripening.PloS one · 2026Article
- Periodontitis-associated Fusobacterium nucleatum promotes ulcerative colitis by ferroptosis-mediated gut barrier disruption.NPJ biofilms and microbiomes · 2025Article
- A long-read sequencing approach to high-resolution profiling of bacterioplankton diversity in a shallow freshwater lake.Scientific reports · 2025Article
- Influence of cervicovaginal microbiota onMicrobial cell (Graz, Austria) · 2025Review
- iMetaOmics: Advancing human and environmental health through integrated meta-omics.iMetaOmics · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria often exist and function as a community, known as the bacterial microbiota, which consists of vast numbers of bacteria belonging to many bacterial species (taxa). Characterizing the bacterial microbiota needs high-throughput approaches that enable the identification and quantification of many bacterial cells, and such approaches have been under development for more than 30 years. In this review, we describe the history of high-throughput technologies based on 16S ribosomal RNA (rRNA) gene-amplicon sequencing for the characterization of bacterial microbiotas. Then, we summarize the features and applications of current 16S rRNA gene-amplicon sequencing approaches, including a recent achievement that enables the identification of individual cells with single-base accuracy for 16S rRNA genes and the quantification of many identified cells. Furthermore, we present the prospects for further technical development, including the combined use of high-throughput methods and other informative analyses, such as whole-genome sequencing in the common unit of the cell, which enables bacterial microbiota characterization based on both the number of cells and their functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.