ArticleResearch square2026
Leukocyte-epithelial physical contacts mediate interstitial migration in vivo.
Jonathan H Schrope, Tanner F Robertson, Adam Horn, Jack J Stevens, Clyde W Tinnen, Julie Rindy, Yiran Hou, Emilie Rochon, David J Beebe, Anna Huttenlocher
Abstract readPreprint
In one paragraphArticle in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0citing papers in PubMed
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
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4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
10 authors.
Jonathan H SchropeDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Tanner F RobertsonDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.
Adam HornDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.
Jack J StevensDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Clyde W TinnenDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Julie RindyDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.ORCID 0000-0002-4242-6427 Yiran HouDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.ORCID 0000-0002-6086-6434 Emilie RochonDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.
David J BeebeDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.ORCID 0000-0002-0415-9006 Anna HuttenlocherDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.ORCID 0000-0001-7940-6254 Funding
UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Justine Yang Bruce · 1985 to 2026
$142.6MCell migration and wound repairR35GM118027 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Anna Huttenlocher · 2016 to 2026
$7.5MBiotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0MUnder-oil open microfluidic system (UOMS) for studying systemic fungal infectionR01AI154940 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BEEBE, DAVID J, MCCLEAN, MEGAN N · 2021 to 2025
$3.8MMOLECULAR ANALYSIS OF HIV1 REVERSE TRANSCRIPTIONR37AI034749 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MORROW, CASEY D · 1999 to 2008
$2.5MA multiplexed micro scale assay for real time analysis of pediatric immune cell functionU24AI152177 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BEEBE, DAVID J, KERR, SHEENA CATHERINE · 2020 to 2024
$1.8MMechanical forces regulate leukocyte migration in rapidly deforming tissuesF32GM146398 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI ROBERTSON, TANNER FORD · 2022 to 2023
$137kInvestigating the role of myeloid-derived growth factor in opposing neutrophil mechanical activation to regulate cardiac healing in the context of myocardial infarctionF30HL174128 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI Jon Schrope · 2024 to 2026
$136kNCI NIH HHS P30 CA014520NHLBI NIH HHS F30 HL174128NIAID NIH HHS R01 AI154940NIAID NIH HHS R37 AI034749NIAID NIH HHS U24 AI152177NIGMS NIH HHS F32 GM146398NIGMS NIH HHS R35 GM118027NIGMS NIH HHS T32 GM135066
6 · The paper itselfAbstract
Efficient immune cell migration requires physical interactions with surrounding tissues. While tissue matrix mechanics influence leukocyte motility, it is unknown how leukocytes exert pushing and pulling forces to traverse tightly adherent epithelial tissues, which comprise a majority of tissue volume in vivo. Here, we leverage the optical transparency of larval zebrafish to identify how physical interactions with epithelial cells regulate mechanisms of neutrophil force generation to navigate cell-dense tissues. Confining forces from epithelial cells induce a mechanosensitive central actin network, mediated by Cdc42 and WASP, which exerts expansile forces on surrounding cells to dilate a path for migration. In concert, direct cell-to-cell (leukocyte-epithelial) contacts, mediated by integrin ɑE binding to epithelial cadherin, generate tractional forces to enable forward motility. Together, our findings identify how physical interactions with surrounding epithelial cells regulate leukocyte motility through cell-dense tissues in vivo.
Identifiers
PMID41674828
PMCPMC12889812
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