ArticleVeterinary medicine and science2026
Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR-Based Quantification of Virus Genomes.
Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR-Based Quantification of Virus Genomes.Veterinary medicine and science · 2026Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative PCR (qPCR) employing either non-specific dyes or gene-specific dye-labelled probes is a commonly used technique for the quantification of viral nucleic acids. While commercial kits often come equipped with their own proprietary standards, many research endeavours necessitate the preparation of bespoke standards that suit the specific needs of the research. This study aimed to assess the appropriateness of various nucleic acid standards, namely circular plasmids, linear plasmids, PCR products, and transcribed RNA, in virus nucleic acid quantification. The evaluation of these standards was conducted through the implementation of qPCR and qRT-PCR methods utilising two different targeted gene-specific primer and probe sets. The findings of this investigation revealed notable differences between the standards. Notably, the study emphasises the necessity to choose standards carefully because their behaviour in PCR is not equal.
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Registered trials
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