ArticleJournal of animal science and biotechnology2026
Potential mechanism of the effect of heat stress on milk protein synthesis revealed by integrated metabolomic and proteomic analyses.
Article in Journal of animal science and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Coated cobalamin alleviates heat stress in lactating Holstein cows by enhancing ruminal propionate production, antioxidant capacity, and hepatic mitogen-activated protein kinase signaling.Journal of animal science and biotechnology · 2026Article
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6 authors.
Funding
Abstract
backgroundThis study was conducted to investigate the impact of varying degrees of heat stress on milk protein synthesis in dairy cows using comprehensive analyses of metabolomics and proteomics. Eighteen dairy cows were subjected to no heat stress (No-HS), mild heat stress (Mild-HS), and moderate heat stress (Mod-HS). Blood and milk samples were collected to determine the content and composition of amino acids (AA), and milk samples were used for metabolomic and proteomic analyses.
resultsMilk protein yield was significantly lower under Mild-HS and Mod-HS than No-HS (P < 0.001). During Mild-HS, no significant difference was found in total AA concentration in both arterial (P = 0.545) and venous blood (P = 0.057), but arterial AA supply to the mammary gland significantly increased (P = 0.045) when compared with No-HS. Under Mod-HS, the supply (P < 0.001) and uptake (P = 0.001) of total AA in the mammary gland decreased significantly, affecting the availability of precursors necessary for milk protein synthesis. Milk metabolomic analysis revealed that Mod-HS significantly impacted nucleotide metabolism, energy metabolism, and protein synthesis processes including translation, folding, and transport. Milk proteomic analysis showed significant downregulation of ribosomal and heat shock proteins which are crucial for protein synthesis and folding.
conclusionsThese findings suggest that heat stress disrupts AA utilization and energy metabolism in the mammary gland, leading to the reduced efficiency in milk protein synthesis and lowered milk protein yield. This study offers valuable insights into the metabolic and proteomic changes in dairy cows under heat stress, highlighting potential strategies to mitigate the adverse effects of heat stress on dairy production and milk quality.
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