Evidence map›Paper›PMID 41673330›Full record

ArticleApplied microbiology and biotechnology2026

Rapid and visual dual-color lateral flow immunoassay for african swine fever virus antibody detection.

Jie Chen, Zhengwang Shi, Yi Ru, Shuaipeng Li, Yuqian Zhu, Juncong Luo, Caixia Jia, Xiaoyang Zhang, Juanjuan Wei, Jing Zhou and 4 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

14 authors.

Jie Chen *State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Zhengwang Shi *State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Yi RuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Shuaipeng LiState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Yuqian ZhuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Juncong LuoState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Caixia JiaState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Xiaoyang ZhangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Juanjuan WeiState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Jing ZhouState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Huanchen LiaoState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Lijuan ZhangSchool of Life Science and Engineering, Northwest Minzu University, LanzhouGansu, 730030, China.
Hong TianState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. xibeitian0931@163.com.ORCID http://orcid.org/0000-0002-1764-4916
Haixue ZhengState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. zhenghaixue@caas.cn.ORCID http://orcid.org/0000-0001-6850-1379

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African swine fever (ASF), an acute infectious disease with a high mortality rate, poses considerable challenges to the development of the global swine industry and socio-economic stability. At present, given the lack of commercial vaccines or effective therapeutic methods, the primary strategy for its management relies on rapid diagnosis and culling of infected animals. Thus, there is a pressing need to develop sensitive, simple, and rapid detection methods. In this study, a latex microsphere (LM)-based dual-color lateral flow immunoassay (LFIA) was designed for the detection of African swine fever virus (ASFV) antibody, which was detected within 15 min by the naked eye. Protein 72 (p72) was conjugated with red LM (RLM) and Chicken IgY (CIgY) with blue LM (BLM) to achieve dual-color detection. Moreover, no cross-reactivity was observed between the standard sera of ASFV and other common swine pathogens. The method exhibited a sensitivity of 1:1024, comparable with the commercial ELISA kit. Meanwhile, the evaluation of 159 clinical samples yielded a kappa value of 0.955. Regarding stability, the assay could be stored at 4℃, room temperature (18-25 ℃), and 37 ℃ for 16, 12, and 10 months, respectively. In addition, the intra- and inter-assay variability showed no significant difference, highlighting the excellent repeatability of the method. Overall, these results demonstrate that the dual-color LM-LFIA offers advantages such as low cost, high sensitivity, strong specificity, outstanding stability, and fast detection speed, making it particularly suitable for point-of-care testing (POCT) of ASF. KEY POINTS: • Visually intuitive: Within 15 min, a red C-line indicates a positive ASFV antibody. • Extremely high accuracy: Compared with commercial ASFV antibody ELISA kit, the Cohen's kappa value of this assay is 0.955. • Comparable sensitivity: The detection limit of this assay is 1:1024, matching the performance of a commercial ASFV ELISA kit.

Indexed as

African Swine FeverAfrican Swine Fever VirusAntibodies, ViralAnimalsCapsid ProteinsEnzyme-Linked Immunosorbent AssayImmunoassayImmunoglobulinsMicrospheresRapid Diagnostic TestsSensitivity and SpecificitySwineAntibodies, Viralcapsid protein p72, African swine fever virusCapsid ProteinsIgYImmunoglobulinsAfrican swine feverDual-color detectionLateral flow immunoassayLatex microsphereP72 protein

Identifiers

PMID41673330
PMCPMC13005836

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