ArticleNPJ precision oncology2026
5-hydroxymethylcytosine profiles in circulating cell-free DNA associate with disease status in patients with osteosarcoma.
Article in NPJ precision oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
- Update of
Authors and funding
10 authors.
Funding
Abstract
5-hydroxymethylcytosine (5-hmC) is a marker of open chromatin and active gene expression. We profiled 5-hmC using plasma-derived cell-free DNA (cfDNA) from patients with osteosarcoma to assess its utility as a biomarker of disease status. Genes with differential 5-hmC levels were identified from a Discovery cohort consisting of patients with osteosarcoma and healthy children. An independent Validation cohort was evaluated using these signature genes. Hierarchical clustering using 262 osteosarcoma signature genes identified in the Discovery cohort identified two clusters of samples in the Validation cohort. Cluster 1 contained 10 of 12 samples from patients with primary disease or osseous metastases, whereas Cluster 2 contained 26 of 33 samples from patients without active disease. Using a semi-quantitative osteosarcoma signature scoring system, the sensitivity and specificity to classify patients with active disease were 65% and 64%, respectively. This technique is feasible in this population, and further investigation with larger patient cohorts is warranted.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.