Evidence map›Paper›PMID 41671182›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2026

Bacterial iron acquisition by

Juanita Lara-Gutiérrez, Jen Nguyen, Matthew R McIlvin, Ichiko Sugiyama, Zachary C Landry, Uria Alcolombri, Sammy Pontrelli, Joaquín Jiménez-Martínez, Uwe Sauer, Terence Hwa and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Iron acquisition by amino acids under flow.Nature reviews. Microbiology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Juanita Lara-GutiérrezInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.ORCID 0000-0003-4555-6988
Jen NguyenInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.ORCID 0000-0002-8286-0738
Matthew R McIlvinMarine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543.
Ichiko SugiyamaMarine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543.
Zachary C LandryInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.
Uria AlcolombriInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.
Sammy PontrelliDepartment of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich 8093, Switzerland.ORCID 0000-0001-6265-8842
Joaquín Jiménez-MartínezInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.ORCID 0000-0002-2063-6490
Uwe SauerDepartment of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich 8093, Switzerland.
Terence HwaDepartment of Physics, University of California at San Diego, La Jolla, CA 92093.
Johannes M KeegstraInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.ORCID 0000-0002-8877-4881
Mak A SaitoMarine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543.ORCID 0000-0001-6040-9295
Roman StockerInstitute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich 8093, Switzerland.ORCID 0000-0002-3199-0508

Funding

Response of the Bacterial Metalloproteome to Environmental ConditionsR01GM135709 · NIGMS · WOODS HOLE OCEANOGRAPHIC INSTITUTION · PI SAITO, MAKOTO · 2020 to 2023
$1.9M
Gordon and Betty Moore Foundation (GBMF) GBMF9197NIGMS NIH HHS R01 GM135709NSF (NSF) 2109890Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) 205321_207488Simons Foundation (SF) 542387FY22Simons Foundation (SF) 542395FY22Simons Foundation (SF) 970834
6 · The paper itself

Abstract

In natural environments, bacteria often encounter low concentrations of nutrient mixtures that are continuously replenished by physical processes such as fluid flow. Studying bacterial physiology under such conditions is experimentally challenging because it is difficult to maintain steady, low nutrient concentrations with rapid renewal. Most studies on nutrient limitation have used approaches such as the chemostat, which rely on long renewal times to sustain low concentrations. We developed a Millifluidic Continuous Culture Device (MCCD), inspired by microfluidics, that enables bacterial cultivation in nutrient mixtures at low micromolar concentrations with rapid renewal driven by fluid flow. Unlike microfluidic systems, the MCCD retains sufficient culture volume to support batch-scale 'omic analyses. Using the MCCD, we cultured

Indexed as

Amino AcidsEscherichia coliIronHistidineSiderophoresAmino AcidsHistidineIronSiderophoresbacterial physiologyiron–amino acid complexesiron uptakeisotope tracinglow-nutrient environment

Identifiers

PMID41671182
PMCPMC12912997

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.