Evidence map›Paper›PMID 41671092›Full record

ArticleSTAR protocols2026

Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei.

Binbin Cui, Xiayu Chen, Mingfang Wang, Yinyue Deng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Binbin CuiPharmacy Department, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo 315046, China; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
Xiayu ChenSchool of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
Mingfang WangSchool of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
Yinyue DengSchool of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China. Electronic address: dengyle@mail.sysu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol to detect the signaling network composed of cyclic AMP (cAMP) and cyclic di-GMP (c-di-GMP) signaling systems in Shigella sonnei. Technical assays include microscale thermophoresis (MST), quantitative reverse-transcription PCR (RT-qPCR), ultra-high-performance liquid chromatography-mass spectrometry (LC-MS), electrophoretic mobility shift assay (EMSA), bacterial adenylate cyclase two-hybrid (BACTH) assay, and molecular docking. For complete details on the use and execution of this protocol, please refer to Wang et al.

Indexed as

Second Messenger SystemsShigella sonneiCyclic AMPCyclic GMPSignal Transductionbis(3',5')-cyclic diguanylic acidCyclic AMPCyclic GMPHealth sciencesMicrobiologyMolecular biology

Identifiers

PMID41671092
PMCPMC12914205

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.