Evidence map›Paper›PMID 41670347›Full record

ArticlemBio2026

An inducible CRISPRi system for phenotypic analysis of essential genes in

Jaryd R Sullivan, Kristina M Ferrara, Rebecca Barrick, Keith P Romano, Thulasi Warrier, Deborah T Hung

Abstract read
In one paragraph

Article in mBio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Oligonucleotide genetics forbioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Jaryd R SullivanDepartment of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.ORCID 0000-0002-8864-8949
Kristina M FerraraDepartment of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.
Rebecca BarrickDepartment of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.
Keith P RomanoDepartment of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.
Thulasi WarrierDepartment of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.ORCID 0000-0002-4384-0873
Deborah T HungDepartment of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.ORCID 0000-0003-4262-0673

Funding

Innovative technologies to transform antibiotic discovery. Project 4 Infection site-specific amplification of antimicrobial conjugatesU19AI142780 · NIAID · BROAD INSTITUTE, INC. · PI HUNG, DEBORAH T · 2019 to 2023
$33.9M
NIAID NIH HHS U19 AI142780
6 · The paper itself

Abstract

Precise and tunable genetic tools are essential for high-throughput functional genomics. To address this need in the important gram-negative pathogen

Indexed as

CRISPR-Cas SystemsGenes, EssentialPseudomonas aeruginosaAnti-Bacterial AgentsGene Expression Regulation, BacterialPhenotypePlasmidsPromoter Regions, GeneticRhamnoseAnti-Bacterial AgentsRhamnoseCRISPRigenetic perturbationPseudomonas aeruginosatranscriptomics

Identifiers

PMID41670347
PMCPMC12977507

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.